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首页> 外文期刊>The European Journal of Neuroscience >Progressive loss of PAX6, TBR2, NEUROD and TBR1 mRNA gradients correlates with translocation of EMX2 to the cortical plate during human cortical development.
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Progressive loss of PAX6, TBR2, NEUROD and TBR1 mRNA gradients correlates with translocation of EMX2 to the cortical plate during human cortical development.

机译：在人类皮质发育过程中，PAX6，TBR2，NEUROD和TBR1 mRNA梯度的逐渐丧失与EMX2向皮质板的转运有关。

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摘要

The transcription factors Emx2 and Pax6 are expressed in the proliferating zones of the developing rodent neocortex, and gradients of expression interact in specifying caudal and rostral identities. Pax6 is also involved in corticoneurogenesis, being expressed by radial glial progenitors that give rise to cells that also sequentially express Tbr2, NeuroD and Tbr1, genes temporally downstream of Pax6. In this study, using in situ hybridization, we analysed the expression of EMX2, PAX6, TBR2, NEUROD and TBR1 mRNA in the developing human cortex between 8 and 12 postconceptional weeks (PCW). EMX2 mRNA was expressed in the ventricular (VZ) and subventricular zones (SVZ), but also in the cortical plate, unlike in the rodent. However, gradients of expression were similar to that of the rodent at all ages studied. PAX6 mRNA expression was limited to the VZ and SVZ. At 8 PCW, PAX6 was highly expressed rostrally but less so caudally, as has been seen in the rodent, however this gradient disappeared early in corticogenesis, by 9 PCW. There was less restricted compartment-specific expression of TBR2, NEUROD and TBR1 mRNA than in the rodent, where the gradients of expression were similar to that of PAX6 prior to 9 PCW. The gradient disappeared for TBR2 by 10 PCW, and for NEUROD and TBR1 by 12 PCW. These data support recent reports that EMX2 but not PAX6 is more directly involved in arealization, highlighting that analysis of human development allows better spatio-temporal resolution than studies in rodents.
机译：转录因子Emx2和Pax6在发育中的啮齿动物新皮层的增生区表达，表达的梯度在指定尾端和鼻端身份时相互作用。 Pax6也参与皮质神经发生，由放射状神经胶质祖细胞表达，该放射状神经胶质祖细胞产生也顺序表达Pax6下游下游基因Tbr2，NeuroD和Tbr1的细胞。在这项研究中，我们使用原位杂交技术，在受孕后8至12周（PCW）之间分析了EMX2，PAX6，TBR2，NEUROD和TBR1 mRNA在发育中的人类皮层中的表达。与啮齿动物不同，EMX2 mRNA在脑室（VZ）和脑室下区域（SVZ）中表达，但也在皮质板中表达。然而，在所有研究的年龄中，表达梯度都与啮齿动物相似。 PAX6 mRNA表达仅限于VZ和SVZ。如在啮齿动物中所见，在8 PCW时，PAX6在额叶中表达较高，但在尾巴中表达较少，但这种梯度在9 PCW的皮质发生早期就消失了。与啮齿类动物相比，TBR2，NEUROD和TBR1 mRNA的限制性区室特异性表达较少，在啮齿类动物中，表达梯度与PAX6在9 PCW之前的相似。 TBR2的梯度消失了10 PCW，NEUROD和TBR1的梯度消失了12 PCW。这些数据支持了最近的报道，即EMX2而非PAX6更直接地参与了区域化，突显了对人类发育的分析比对啮齿类动物的研究具有更好的时空分辨率。

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