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首页> 外文期刊>The European Journal of Neuroscience >Correlation between electrophysiological and morphological characteristics during maturation of rat supraoptic neurons.
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Correlation between electrophysiological and morphological characteristics during maturation of rat supraoptic neurons.

机译：大鼠超视神经元成熟期间电生理和形态特征之间的相关性。

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摘要

The neurohypophysial peptides oxytocin (OT) and vasopressin (AVP) are well known for their role in reproductive functions and fluid balance regulation, respectively. During development, these peptides are thought to act as trophic factors on both peripheral and central structures. However, despite this early developmental function, the maturation of their secreting neurons remains poorly investigated. In this study, we have characterized the electrical and morphological characteristics displayed by OT and AVP supraoptic (SO) neurons between embryonic day 21 and postnatal day 20. Transient changes in passive membrane properties, correlated with a transient increase in the dendritic arborization, were observed at the beginning of the second postnatal week (PW2). The action potential matured mostly during PW1 and its threshold progressively hyperpolarized in parallel with the resting membrane potential. During PW1, SO neurons displayed unique characteristics with a low-threshold Ca(2+)-dependent depolarizing potential and a prominent hyperpolarization-activated current (I(h) ). This latter is involved in a depolarizing sag during hyperpolarization and an after hyperpolarizing potential following a depolarization. During this period, maintaining E(Cl) unchanged by the use of gramicidin-perforated patch recordings revealed excitatory GABAergic potentials, that became inhibitory during PW2, whilst glutamatergic potential appeared. The electrical activity was very erratic in young neurons and progressively differentiated in the typical firing observed in mature neurons (tonic and phasic for OT and AVP neurons, respectively) during PW2--3. These results show that the development of electrical properties of SO neurons is correlated with the maturation of their dendritic arborization.
机译：神经下体肽催产素（OT）和加压素（AVP）分别因其在生殖功能和体液平衡调节中的作用而闻名。在开发过程中，这些肽被认为在外周和中央结构上均充当营养因子。然而，尽管具有这种早期发育功能，但其分泌神经元的成熟仍然研究不足。在这项研究中，我们表征了在胚胎第21天至出生后第20天之间，OT和AVP超视（SO）神经元显示的电学和形态学特征。观察到被动膜特性的瞬时变化与树突状树突的瞬时增加相关。在产后第二个星期（PW2）开始时。动作电位主要在PW1期间成熟，其阈值与静止膜电位平行地逐渐超极化。在PW1期间，SO神经元显示出具有低阈值Ca（2+）依赖的去极化电位和突出的超极化激活电流（I（h））的独特特征。后者涉及超极化过程中的去极化流挂以及去极化后的超极化电位。在此期间，通过使用短杆菌肽穿孔的贴片录音保持E（Cl）不变，可显示出兴奋性GABA能电位，该电位在PW2期间被抑制，而谷氨酸能出现。在PW2--3期间，年轻神经元的电活动非常不稳定，并在成熟神经元中观察到的典型放电中逐渐分化（分别为OT和AVP神经元为声调和相声）。这些结果表明，SO神经元电学特性的发展与其树突状树突的成熟有关。

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