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首页> 外文期刊>The European Journal of Neuroscience >Migration of cells into and out of peripheral nerve isografts in the peripheral and central nervous systems of the adult mouse.
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Migration of cells into and out of peripheral nerve isografts in the peripheral and central nervous systems of the adult mouse.

机译：成年小鼠外周和中枢神经系统中细胞向周围神经异种移植物中的迁移和迁移。

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摘要

Peripheral nerve (PN) isografts provide a favourable environment for axon regeneration after peripheral and central nervous system (CNS) injury, but definitive information on the extent of cellular intermixing between donor and host tissues is lacking. We wished to compare migration patterns in fresh and predegenerate PN grafts, and also compare the extent of cell migration after transplantation to peripheral nervous system (PNS) versus CNS. To discern how host and donor cells interact after PN transplantation, sciatic nerve segments were transplanted from inbred adult mice into PN defects (PN-PN grafts) or into lesioned cerebral cortex of opposite gender siblings. Migrating male cells were identified using a Y-chromosome-specific probe and in situ hybridization methods, and characterized immunohistochemically. The extent of donor and host cellular intermixing was similar in fresh and predegenerate PN-PN isografts. There was substantial intermixing of donor and host cells by 8 days. Many host cells migrating into epineurial regions of grafts were immunopositive for F4/80 (macrophages). The endoneurium of grafted PN was also colonized by host cells; some were F4/80+ but many were immunostained with S-100 (Schwann cell marker). Donor S-100+ Schwann cells rapidly migrated out into proximal and distal host PN and by 12 weeks were found at least 2 mm from the grafts. Endoneurial microvessels in grafts were mostly donor-derived. By comparison, in male PN grafts to female CNS, even after 6 weeks few donor cells had migrated out into surrounding host cortex, despite the observation that almost all grafts contained regenerating axons and were thus attached to host CNS tissue.
机译：周围神经（PN）同种异体移植为周围和中枢神经系统（CNS）损伤后的轴突再生提供了良好的环境，但缺乏有关供体和宿主组织之间细胞混合程度的确切信息。我们希望比较新鲜和退化过的PN移植物中的迁移模式，还希望比较移植到周围神经系统（PNS）与CNS后的细胞迁移程度。为了辨别PN移植后宿主细胞和供体细胞之间的相互作用，从近交成年小鼠的坐骨神经节段移植到PN缺损（PN-PN移植）或异性同胞同胞的病变皮层中。使用Y染色体特异性探针和原位杂交方法鉴定迁移的雄性细胞，并进行免疫组织化学鉴定。在新鲜和简并的PN-PN同种异体移植物中，供体和宿主细胞混合的程度相似。到8天，供体和宿主细胞充分混合。迁移到移植物肾上腺区的许多宿主细胞对F4 / 80（巨噬细胞）免疫阳性。移植的PN的神经内膜也被宿主细胞定殖。一些是F4 / 80 +，但许多是用S-100（Schwann细胞标记）免疫染色的。供体S-100 +雪旺细胞迅速迁移到近端和远端宿主PN中，并在距移植物至少2 mm处发现了12周。移植物中的神经内膜微血管大多来自供体。相比之下，在雄性PN移植到雌性CNS的过程中，尽管观察到几乎所有移植物都包含再生轴突并因此附着在宿主CNS组织上，但是即使经过6周，几乎没有供体细胞迁移到周围的宿主皮层中。

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