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首页> 外文期刊>The European Journal of Neuroscience >Parvalbumin-producing cortical interneurons receive inhibitory inputs on proximal portions and cortical excitatory inputs on distal dendrites
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Parvalbumin-producing cortical interneurons receive inhibitory inputs on proximal portions and cortical excitatory inputs on distal dendrites

机译：产生小白蛋白的皮质中间神经元在近端接受抑制性输入，而在远端树突上接受皮质兴奋性输入。

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摘要

To examine inputs to parvalbumin (PV)-producing interneurons, we generated transgenic mice expressing somatodendritic membrane-targeted green fluorescent protein specifically in the interneurons, and completely visualized their dendrites and somata. Using immunolabeling for vesicular glutamate transporter (VGluT)1, VGluT2, and vesicular GABA transporter, we found that VGluT1-positive terminals made contacts 4- and 3.1-fold more frequently with PV-producing interneurons than VGluT2-positive and GABAergic terminals, respectively, in the primary somatosensory cortex. Even in layer 4, where VGluT2-positive terminals were most densely distributed, VGluT1-positive inputs to PV-producing interneurons were 2.4-fold more frequent than VGluT2-positive inputs. Furthermore, although GABAergic inputs to PV-producing interneurons were as numerous as VGluT2-positive inputs in most cortical layers, GABAergic inputs clearly preferred the proximal dendrites and somata of the interneurons, indicating that the sites of GABAergic inputs were more optimized than those of VGluT2-positive inputs. Simulation analysis with a PV-producing interneuron model compatible with the present morphological data revealed a plausible reason for this observation, by showing that GABAergic and glutamatergic postsynaptic potentials evoked by inputs to distal dendrites were attenuated to 60 and 87%, respectively, of those evoked by somatic inputs. As VGluT1-positive and VGluT2-positive axon terminals were presumed to be cortical and thalamic glutamatergic inputs, respectively, cortical excitatory inputs to PV-producing interneurons outnumbered the thalamic excitatory and intrinsic inhibitory inputs more than two-fold in any cortical layer. Although thalamic inputs are known to evoke about two-fold larger unitary excitatory postsynaptic potentials than cortical ones, the present results suggest that cortical inputs control PV-producing interneurons at least as strongly as thalamic inputs.
机译：为了检查产生小白蛋白（PV）的中间神经元的输入，我们生成了在中间神经元中特异性表达体树突状膜靶向绿色荧光蛋白的转基因小鼠，并完全可视化了它们的树突和躯体。通过对囊泡谷氨酸转运蛋白（VGluT）1，VGluT2和囊泡GABA转运蛋白进行免疫标记，我们发现VGluT1阳性末端与产生PV的中子的接触频率分别比VGluT2阳性和GABA阳性末端高4到3.1倍，在主要的体感皮质中。即使在VGluT2阳性端子分布最密集的第4层中，产生PV的中间神经元的VGluT1阳性输入的频率也比VGluT2阳性输入的频率高2.4倍。此外，尽管在大多数皮质层中，产生PV的中间神经的GABA能输入与VGluT2阳性输入一样多，但GABA能输入显然更倾向于中间神经的近端树突和体细胞，这表明GABA能输入的位置比VGluT2更优化。 -正输入。用PV产生的中间神经元模型与当前形态学数据兼容的模拟分析揭示了该观察结果的合理原因，这表明远端树突的输入所诱发的GABA能和谷氨酸能突触后突触电位分别被衰减至所诱发的60％和87％。通过体细胞输入。由于VGluT1阳性和VGluT2阳性轴突末端分别被认为是皮质和丘脑的谷氨酸能输入，因此在任何皮质层中，产生PV的中间神经的皮质兴奋性输入超过丘脑兴奋性和固有抑制性输入。尽管已知丘脑输入能激发比皮质皮质大两倍的单位兴奋性突触后突触电位，但目前的结果表明皮质输入至少能像丘脑输入一样强烈地控制产生PV的神经元。

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