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首页> 外文期刊>The European Journal of Neuroscience >Site-specific effects of gastrin-releasing peptide in the suprachiasmatic nucleus
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Site-specific effects of gastrin-releasing peptide in the suprachiasmatic nucleus

机译：胃泌素上核中胃泌素释放肽的位点特异性效应

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摘要

The effects of gastrin-releasing peptide (GRP) on the circadian clock in the suprachiasmatic nucleus (SCN) are dependent on the activation of N-methyl-d-aspartate (NMDA) receptors in the SCN. In this study, the interaction between GRP, glutamate and serotonin in the regulation of circadian phase in Syrian hamsters was evaluated. Microinjection of GRP into the third ventricle induced c-fos and p-ERK expression throughout the SCN. Coadministration of an NMDA antagonist or 8-hydroxy-2-di-n-propylamino-tetralin [a serotonin (5-HT)1A,7 agonist, DPAT] with GRP limited c-fos expression in the SCN to a region dorsal to GRP cell bodies. Similar to the effects of NMDA antagonists, DPAT attenuated GRP-induced phase shifts in the early night, suggesting that the actions of serotonin on the photic phase shifting mechanism occur downstream from retinorecipient cells. c-fos and p-ERK immunoreactivity in the supraoptic (SON) and paraventricular hypothalamic nuclei also increased following ventricular microinjection of GRP. Because of this finding, a second set of experiments was designed to test a potential role for the SON in the regulation of clock function. Syrian hamsters were given microinjections of GRP into the peri-SON during the early night. GRP-induced c-fos activity in the SCN was similar to that following ventricular administration of GRP. GRP or bicuculline (a γ-aminobutyric acidA antagonist) administered near the SON during the early night elicited phase delays of circadian activity rhythms. These data suggest that GRP-induced phase-resetting is dependent on levels of glutamatergic and serotonergic neurotransmission in the SCN and implicate activity in the SON as a potential regulator of photic signaling in the SCN.
机译：胃泌素释放肽（GRP）对视交叉上核（SCN）中昼夜节律的影响取决于SCN中N-甲基-d-天冬氨酸（NMDA）受体的激活。在这项研究中，评估了GRP，谷氨酸和5-羟色胺在叙利亚仓鼠昼夜节律调控中的相互作用。在整个SCN中向第三脑室显微注射GRP诱导c-fos和p-ERK表达。 NMDA拮抗剂或8-羟基-2-二-正丙基氨基四氢萘[5-羟色胺（5-HT）1A，7激动剂，DPAT]与GRP在SCN中的c-fos表达共同限制在GRP背侧细胞体。与NMDA拮抗剂的作用类似，DPAT在深夜减弱了GRP诱导的相移，这表明5-羟色胺对光相移机制的作用发生在视网膜受体细胞的下游。脑室显微注射GRP后，视上（SON）和室下丘脑核中的c-fos和p-ERK免疫反应性也增加。由于这一发现，设计了第二组实验，以测试SON在调节时钟功能中的潜在作用。在清晨，向叙利亚仓鼠显微注射了GRP到peri-SON。 GRP诱导的SCN中c-fos活性类似于心室给予GRP后的活性。在深夜在SON附近使用GRP或双瓜氨酸（一种γ-氨基丁酸A拮抗剂）会引起生理活动节律的相位延迟。这些数据表明，GRP诱导的相位重置取决于SCN中的谷氨酸能和5-羟色胺能神经传递水平，并且在SON中具有作为SCN潜在的光信号调节剂的功能。

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