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首页> 外文期刊>The European Journal of Neuroscience >Astrocyte-endothelial cell calcium signals conveyed by two signalling pathways.
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Astrocyte-endothelial cell calcium signals conveyed by two signalling pathways.

机译：星形胶质细胞-内皮细胞钙信号通过两个信号通路传递。

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摘要

Astrocytes and endothelial cells are in close contact with each other at the blood-brain barrier, where important molecular transports take place. Despite these key morphological and functional properties, little is known regarding the dynamic signalling processes that occur between these two cell types. We investigated astrocyte-endothelial cell calcium signalling mechanisms in a coculture model prepared from primary rat cortical astrocytes and ECV304 cells. We used flash photolysis of caged inositol-trisphosphate (IP3) and gentle mechanical stimulation to trigger astrocyte-endothelial cell calcium signals and to investigate the underlying propagation mechanisms. Photolytically releasing IP3 in a single cell triggered increases in cytoplasmic calcium concentration that propagated between astrocytes and endothelial cells in either direction. These propagating calcium signals did not cross cell-free zones and were not affected by fast superfusion or by the purinergic inhibitors apyrase and suramin, indicating that they are communicated through an intracellular pathway in conjunction with gap junctions. Electrophysiological experiments confirmed a low degree of astrocyte-endothelial cell electrical cell-to-cell coupling. Mechanical stimulation of a single cell also triggered astrocyte-endothelial cell calcium signals but, in contrast to the former triggering mode, these signals crossed cell-free zones and were significantly inhibited by apyrase, thus indicating the involvement of an extracellular and purinergic messenger. Astrocyte-endothelial cell calcium signalling also occurred in cocultures prepared with astrocytes and primary rat brain capillary endothelial cells. We conclude that astrocytes and endothelial cells can exchange fast-acting calcium signals (time scale of seconds) that can be communicated through an intracellular/gap junctional pathway and an extracellular purinergic pathway.
机译：星形胶质细胞和内皮细胞在血脑屏障处彼此紧密接触，在那里发生重要的分子转运。尽管具有这些关键的形态和功能特性，但对于在这两种细胞类型之间发生的动态信号传导过程知之甚少。我们在从原代大鼠皮质星形胶质细胞和ECV304细胞制备的共培养模型中研究了星形胶质细胞-内皮细胞钙信号传导机制。我们使用了笼型肌醇三磷酸（IP3）的快速光解和轻柔的机械刺激来触发星形胶质细胞-内皮细胞钙信号并研究潜在的传播机制。在单个细胞中光解释放IP3会触发星形胶质细胞和内皮细胞在任一方向上传播的胞质钙浓度增加。这些传播的钙信号没有越过无细胞区，也不受快速灌注或嘌呤能抑制剂腺苷三磷酸双磷酸酶和苏拉明的影响，表明它们通过与间隙连接的细胞内途径进行通讯。电生理实验证实，星形胶质细胞与内皮细胞之间的电细胞耦合程度较低。机械刺激单个细胞也会触发星形胶质细胞-内皮细胞的钙信号，但与以前的触发模式相反，这些信号穿过无细胞区并被腺苷三磷酸酶显着抑制，因此表明细胞外和嘌呤能使者参与其中。星形胶质细胞-内皮细胞钙信号转导也发生在与星形胶质细胞和原代大鼠脑毛细血管内皮细胞制备的共培养物中。我们得出的结论是，星形胶质细胞和内皮细胞可以交换可通过细胞内/间隙连接途径和细胞外嘌呤能途径传递的速效钙信号（时间尺度为秒）。

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