...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Ectopic expression of the neural cell adhesion molecule L1 in astrocytes leads to changes in the development of the corticospinal tract.
【24h】

Ectopic expression of the neural cell adhesion molecule L1 in astrocytes leads to changes in the development of the corticospinal tract.

机译：神经细胞粘附分子L1在星形胶质细胞中的异位表达导致皮质脊髓束发育的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cell recognition molecule L1, of the immunoglobulin superfamily, participates in the formation of the nervous system and has been shown to enhance cell migration and neurite outgrowth in vitro. To test whether ectopic expression of L1 would influence axonal outgrowth in vivo, we studied the development of the corticospinal tract in transgenic mice expressing L1 in astrocytes under the control of the GFAP-promoter. Corticospinal axons innervate their targets by extending collateral branches interstitially along the axon shaft following a precise spatio-temporal pattern. Using DiI as an anterograde tracer, we found that in the transgenic animals, corticospinal axons appear to be defasciculated, reach their targets sooner and form collateral branches innervating the basilar pons at earlier developmental stages and more diffusely than in wild type littermates. Collateral branches in the transgenic mice did not start out as distinct rostral and caudal sets, but they branched from the axon segments in a continuous rostrocaudal direction across the entire region of the corticospinal tract overlying the basilar pons. The ectopic branches are transient and no longer present at postnatal day 22. The earlier outgrowth and altered branching pattern of corticospinal axons in the transgenics is accompanied by an earlier differentiation of astrocytes. Taken together, our observations provide evidence that the ectopic expression of L1 on astrocytes causes an earlier differentiation of these cells, results in faster progression of corticospinal axons and influences the branching pattern of corticospinal axons innervating the basilar pons.
机译：免疫球蛋白超家族的细胞识别分子L1参与神经系统的形成，并已显示出可在体外增强细胞迁移和神经突向外生长。为了测试L1的异位表达是否会影响体内轴突生长，我们研究了在GFAP启动子控制下，星形胶质细胞中表达L1的转基因小鼠的皮质脊髓束的发育。皮质脊髓轴突通过沿精确的时空模式沿轴突轴间隙地扩展侧支分支来支配其目标。使用DiI作为顺行示踪剂，我们发现在转基因动物中，皮质脊髓轴突似乎已消失，可以更快地到达其靶标，并形成了在早期发育阶段支配基底桥的侧支，并且比野生型同窝幼虫更分散。转基因小鼠中的侧支并不以独特的鼻侧和尾侧集开始，而是从轴突段沿连续的尾脑尾方向分支到覆盖基底桥的整个皮质脊髓区域。异位分支是短暂的，在出生后第22天不再存在。在转基因中皮质脊髓轴突的早期生长和分支模式的改变伴随着星形胶质细胞的早期分化。综上所述，我们的观察提供了证据，即星形胶质细胞上L1的异位表达导致这些细胞的早期分化，导致皮质脊髓轴突的发展更快，并影响了支配基底桥的皮质脊髓轴突的分支模式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号