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首页> 外文期刊>The European Journal of Neuroscience >Radial migration in the cerebral cortex is enhanced by signals from thalamus.
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Radial migration in the cerebral cortex is enhanced by signals from thalamus.

机译：丘脑信号增强了大脑皮层的径向迁移。

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The six layered cerebral cortex derives from cells that divide in the ventricular zone and migrate to their final destination in the cortical plate (future cortex). In the mouse, cortical layer III and IV neurons undergo their final mitotic division at around E16, at which time thalamic axons are beginning to enter the cortex. We used bromodeoxyuridine-birth dating of cells in cortical slice cultures to show that the thalamus enhances the migration out of the ventricular zone of future layer III/IV cells. When cortical slices were cultured alone, less than 35% of cells born in vitro on E16 were present in the pial half of the slice after 48 h in culture. In contrast, when cortical slices were cocultured with thalamus, 69% of these cells were found in the pial half of the slice. Explants of other developing tissues did not mimic the effect of the thalamus. The thalamus had no obvious effect on cortical radial glial cells, cortical cell viability or maintenance of cortical slice structure. We found that most precursors born at a similar age but in vivo, shortly before cortical slices were isolated, migrated to the pial half of the cultured slices in the absence of a cocultured thalamic explant. Thus, E16 cortical slices cultured without thalamus permit migration of cells born in vivo and therefore already exposed to the thalamus. Our results indicate that the thalamus provides factors to E16-born cortical precursors that enhance their directed migration out of the ventricular zone to the cortical plate.
机译：六层大脑皮层源自在心室区分裂并迁移到其最终位置的皮质板（未来皮层）中的细胞。在小鼠中，皮质的III和IV层神经元在E16左右经历了最终的有丝分裂分裂，此时丘脑轴突开始进入皮质。我们在皮质切片培养物中使用溴脱氧尿嘧啶核苷的出生日期来证明丘脑增强了未来III / IV层细胞从心室区的迁移。当单独培养皮质切片时，在培养48小时后，不到35％的E16体外出生的细胞存在于切片的半个部分中。相反，当将皮质切片与丘脑共培养时，这些细胞的69％在切片的半透明部分中发现。其他发育中的组织的外植体不能模仿丘脑的作用。丘脑对皮层径向胶质细胞，皮层细胞活力或皮层切片结构的维持没有明显影响。我们发现，在没有皮层丘脑外植体的情况下，在分离皮层片之前不久，大多数前体在相似的年龄但在体内出生，迁移到培养片的半部分。因此，在没有丘脑的情况下培养的E16皮质切片允许体内出生的细胞迁移，因此已经暴露于丘脑。我们的结果表明，丘脑为E16出生的皮质前体提供了因素，从而增强了它们从心室区向皮质板的定向迁移。

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