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首页> 外文期刊>The European Journal of Neuroscience >Adaptive and non-adaptive changes in activity-deprived presynaptic terminals
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Adaptive and non-adaptive changes in activity-deprived presynaptic terminals

机译：缺乏活动的突触前终末期的适应性和非适应性变化

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How the number of docked vesicles is regulated is still unclear. Following chronic activity blockade the number of docked vesicles increases, providing a model through which to address this issue. We tested the hypotheses that the number of docked vesicles is regulated with the size of the terminal, and by the level of Rab3-interacting molecule 1/2 (RIM1/2). We immobilized mouse hippocampal slice cultures by high-pressure freezing after 3 days of tetrodotoxin treatment and analysed them by electron microscopy. The number of docked vesicles, the size of the active zones and the amount of GluA2 were increased after activity blockade. However, there was no modification of either the total number of synaptic vesicles or the area of presynaptic profiles. Surprisingly, immunocytochemistry showed no change in the mean level of RIM1/2 per terminal but its distribution was modified. Additionally, there was no modification of the mean frequency or amplitude of miniature excitatory postsynaptic currents, but the distribution of amplitudes was modified. These results indicate a specific homeostatic regulation of the synaptic junction. The number of docked vesicles does not seem to be regulated by the amount of RIM1/2. The modification of the distribution, but not the amount, of RIM1/2 may explain the contradiction between the morphological and electrophysiological findings.
机译：尚不清楚如何调节对接囊泡的数量。慢性活动受阻后，对接囊泡的数量增加，从而提供了解决此问题的模型。我们测试了这样的假设，即停泊的囊泡的数量受末端大小以及与Rab3相互作用的分子1/2（RIM1 / 2）的水平调控。我们在河豚毒素处理3天后通过高压冷冻固定了小鼠海马切片培养物，并通过电子显微镜对其进行了分析。活性阻断后，对接囊泡的数量，活性区的大小和GluA2的量均增加。但是，无论是突触小泡的总数还是突触前分布的面积都没有改变。出人意料的是，免疫细胞化学显示每端RIM1 / 2的平均水平没有变化，但是其分布被改变了。另外，微型兴奋性突触后电流的平均频率或幅度没有改变，但幅度的分布得到了修正。这些结果表明突触连接的特定稳态调节。停泊的囊泡的数量似乎不受RIM1 / 2量的调节。 RIM1 / 2分布的改变而不是数量的改变可以解释形态学和电生理学发现之间的矛盾。

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