...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Cajal-Retzius cells in early postnatal mouse cortex selectively express functional metabotropic glutamate receptors.
【24h】

Cajal-Retzius cells in early postnatal mouse cortex selectively express functional metabotropic glutamate receptors.

机译：出生后早期小鼠皮质的Cajal-Retzius细胞选择性表达功能性代谢型谷氨酸受体。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glutamate receptors have been linked to the regulation of several developmental events in the CNS. By using cortical slices of early postnatal mice, we show that in layer I cells, glutamate produces intracellular calcium ([Ca(2+)](i)) elevations mediated by ionotropic and metabotropic glutamate receptors (mGluRs). The contribution of mGluRs to these responses was demonstrated by application of tACPD, an agonist to groups I and II mGluRs, which evoked [Ca(2+)](i) increases that could be reversibly blocked by MCPG, an antagonist to groups I and II mGluRs. In the absence of extracellular Ca(2+), repetitive applications of tACPD or quisqualate, an agonist to group I mGluRs, elicited decreasing [Ca(2+)](i) responses that were restored by refilling a thapsigargin-sensitive Ca(2+) store. The use of specific group I mGluR agonists CHPG and DHPG indicated that the functional mGluR in layer I was of the mGluR1 subtype. Subtype specific antibodies confirmed the presence of mGlur1 alpha, but not mGluR5, in Cajal-Retzius (Reelin-immunoreactive) neurons.
机译：谷氨酸受体与中枢神经系统中几种发育事件的调节有关。通过使用早期产后小鼠的皮质切片，我们显示，在第I层细胞中，谷氨酸产生了由离子型和代谢型谷氨酸受体（mGluRs）介导的细胞内钙（[Ca（2 +）]（i））升高。 mGluRs对这些反应的贡献通过应用tACPD（第I和第II组mGluRs的激动剂）得到证明，它引起[Ca（2 +）]（i）升高，而MCPG是第I和第II组的拮抗剂可逆转阻断II mGluR。在缺少细胞外Ca（2+）的情况下，重复应用tACPD或喹喹，一种对第I组mGluRs的激动剂，可引起[Ca（2 +）]（i）应答降低，这种应答可通过补充thapsigargin敏感的Ca（2）恢复。 +）商店。使用特定的第I组mGluR激动剂CHPG和DHPG表明，第I层中的功能性mGluR具有mGluR1亚型。亚型特异性抗体证实在Cajal-Retzius（Reelin免疫反应性）神经元中存在mGlur1 alpha，但不存在mGluR5。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号