...
首页> 外文期刊>The European Journal of Neuroscience >Novel fast adapting interneurons mediate cholinergic-induced fast GABA_A inhibitory postsynaptic currents in striatal spiny neurons
【24h】

Novel fast adapting interneurons mediate cholinergic-induced fast GABA_A inhibitory postsynaptic currents in striatal spiny neurons

机译:新型快速适应性神经元介导纹状棘状神经元中胆碱能诱导的快速GABA_A抑制突触后电流。

获取原文
获取原文并翻译 | 示例
           

摘要

Previous work suggests that neostriatal cholinergic interneurons control the activity of several classes of GABAergic interneurons through fast nicotinic receptor-mediated synaptic inputs. Although indirect evidence has suggested the existence of several classes of interneurons controlled by this mechanism, only one such cell type, the neuropeptide-Y-expressing neurogliaform neuron, has been identified to date. Here we tested the hypothesis that in addition to the neurogliaform neurons that elicit slow GABAergic inhibitory responses, another interneuron type exists in the striatum that receives strong nicotinic cholinergic input and elicits conventional fast GABAergic synaptic responses in projection neurons. We obtained in vitro slice recordings from double transgenic mice in which Channelrhodopsin-2 was natively expressed in cholinergic neurons and a population of serotonin receptor-3a-Cre-expressing GABAergic interneurons were visualized with tdTomato. We show that among the targeted GABAergic interneurons a novel type of interneuron, termed the fast-adapting interneuron, can be identified that is distinct from previously known interneurons based on immunocytochemical and electrophysiological criteria. We show using optogenetic activation of cholinergic inputs that fast-adapting interneurons receive a powerful supra-threshold nicotinic cholinergic input in vitro. Moreover, fast adapting neurons are densely connected to projection neurons and elicit fast, GABA_A receptor-mediated inhibitory postsynaptic current responses. The nicotinic receptor-mediated activation of fast-adapting interneurons may constitute an important mechanism through which cholinergic interneurons control the activity of projection neurons and perhaps the plasticity of their synaptic inputs when animals encounter reinforcing or otherwise salient stimuli.
机译:先前的工作表明,新纹状体胆碱能中间神经元通过快速的烟碱样受体介导的突触输入来控制几类GABA能神经元的活性。尽管间接证据表明存在由这种机制控制的几类中间神经元,但迄今为止,仅鉴定出一种类型的细胞,即表达神经肽-Y的神经胶质样神经元。在这里,我们测试了这样的假设:除了神经胶质样神经元引起缓慢的GABA能抑制反应外,纹状体中还存在另一种中间神经元类型,该神经元接收强的烟碱胆碱能输入并在投射神经元中引起常规的快速GABA能突触反应。我们从双转基因小鼠中获得体外切片记录,其中在胆碱能神经元中天然表达Channelrhodopsin-2,并用tdTomato可视化表达5-羟色胺受体3a-Cre的GABA能性中神经元。我们显示,在靶向的GABA能性神经元中,一种新型的神经元,称为快速适应性神经元,可以根据免疫细胞化学和电生理学标准与先前已知的神经元区别开来。我们显示使用胆碱能输入的光遗传学激活,快速适应的中间神经元在体外会收到强大的超阈值烟碱胆碱能输入。此外,快速适应的神经元紧密连接到投射神经元,并引起快速的GABA_A受体介导的抑制性突触后电流反应。烟碱受体介导的快速适应性神经元的激活可能构成了重要的机制,通过这种机制,胆碱能神经元可以控制投射神经元的活动,并在动物遇到增强刺激或其他显着刺激时控制其突触输入的可塑性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号