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首页> 外文期刊>The European Journal of Neuroscience >Functional characterization of a sex pheromone receptor in the pest moth Spodoptera littoralis by heterologous expression in Drosophila
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Functional characterization of a sex pheromone receptor in the pest moth Spodoptera littoralis by heterologous expression in Drosophila

机译：通过在果蝇中的异源表达对害虫蛾斜纹夜蛾中性信息素受体的功能表征

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Moth sex pheromone communication is recognised as a long-standing model for insect olfaction studies, and a widespread knowledge has been accumulated on this subject thanks to numerous chemical, electrophysiological and behavioural studies. A key step has been the identification of candidate sex pheromone receptors, opening new routes to understanding the specificity and sensitivity of this communication system, but only few of these receptors have as yet been functionally characterised. In this context, we aim at unravelling the molecular bases of pheromone reception in the noctuid moth Spodoptera littoralis. Taking advantage of a collection of antennal-expressed sequence tags, we previously identified three fragments of candidate pheromone receptors in this species. Here, we report full-length cloning of one of these receptors, named SlitOR6. Both sequence and expression pattern analyses were consistent with its annotation as a pheromone receptor, which we further confirmed by functional characterization. Using Drosophila antennae as a heterologous expression system, we identified a single component of the pheromone blend of S. littoralis, (Z,E)-9,12-tetradecadienyl acetate, as the ligand of SlitOR6. Two strategies were employed: (i) expressing SlitOR6 in the majority of Drosophila olfactory neurons, in addition to endogenous receptors, and monitoring the responses to pheromone stimuli by electroantennography; (ii) replacing the Drosophila pheromone receptor OR67d with SlitOR6 and monitoring the response by single sensillum recordings. Results were fully congruent and responses to (Z,E)-9,12-tetradecadienyl acetate were highly specific in both heterologous systems. This approach appears to be efficient and reliable for studying moth pheromone receptors in an in vivo context.
机译：蛾性信息素通讯被认为是昆虫嗅觉研究的长期模型，由于进行了许多化学，电生理和行为研究，该学科已积累了广泛的知识。关键步骤是鉴定候选性信息素受体，为理解该通讯系统的特异性和敏感性开辟了新途径，但这些受体中只有少数在功能上有所表征。在这种情况下，我们旨在揭示夜蛾蛾斜纹夜蛾中信息素接收的分子基础。利用天线表达的序列标签的集合，我们以前确定了该物种中候选信息素受体的三个片段。在这里，我们报道了一种名为SlitOR6的受体的全长克隆。序列和表达模式分析均与其作为信息素受体的注释一致，我们通过功能表征进一步证实了这一点。使用果蝇触角作为异源表达系统，我们确定了S. littoralis（Z，E）-9,12-十四碳二烯基乙酸酯的信息素共混物的单个组分，作为SlitOR6的配体。采用了两种策略：（i）除内源性受体外，还在大多数果蝇嗅觉神经元中表达SlitOR6，并通过电子血管造影术监测对信息素刺激的反应; （ii）用SlitOR6代替果蝇信息素受体OR67d，并通过单次sensillum记录监测反应。结果是完全一致的，并且在两个异源系统中对（Z，E）-9,12-十四碳二烯基乙酸酯的响应都是高度特异性的。这种方法对于在体内研究蛾信息素受体似乎是有效和可靠的。

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