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首页> 外文期刊>The European Journal of Neuroscience >GABA(A)-receptor expression in glioma cells is triggered by contact with neuronal cells.
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GABA(A)-receptor expression in glioma cells is triggered by contact with neuronal cells.

机译：神经胶质瘤细胞中的GABA（A）受体表达是通过与神经元细胞接触而触发的。

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摘要

The expression of functional GABA(A)-receptors in glioma cells correlates with low malignancy of tumours and cell lines from glioma lack these receptors. Here we show that contact with neurons induces the expression of functional GABA(A)-receptors. C6 and F98 glioma cell lines were labelled by recombinant expression of enhanced green fluorescent protein injected into rat brain and studied in acute slices after two to three weeks of tumour growth. The cells responded to GABA or the specific agonist, muscimol with a current typical for GABA(A)-receptors, as studied with the patch-clamp technique. To get insight into the mechanism of GABA(A) receptor induction, the C6 or F98 cells were co-cultured with neurons, astrocytes, oligodendrocytes and microglia. Glioma cells expressed functional GABA(A) receptors within 24 h only in cultures where physical contact to neurons occurred. Activation of GABA(A)-receptors in the co-cultures attenuated glioma cell metabolism while blockade of the receptors increased metabolism. We conclude that with this form of interaction, neurons can influence tumour behaviour in the brain.
机译：神经胶质瘤细胞中功能性GABA（A）受体的表达与肿瘤的低恶性相关，来自神经胶质瘤的细胞系缺乏这些受体。在这里，我们显示与神经元的接触诱导功能性GABA（A）受体的表达。 C6和F98胶质瘤细胞系通过重组表达的绿色荧光蛋白注射到大鼠脑中进行标记，并在肿瘤生长两到三周后在急性切片中进行研究。用膜片钳技术研究，细胞对GABA或特定的激动剂麝香酚具有GABA（A）受体的典型电流。为了深入了解GABA（A）受体的诱导机制，将C6或F98细胞与神经元，星形胶质细胞，少突胶质细胞和小胶质细胞共培养。胶质瘤细胞仅在与神经元发生物理接触的培养物中在24小时内表达功能性GABA（A）受体。共培养物中GABA（A）受体的激活减弱了胶质瘤细胞的新陈代谢，而受体的阻断则增加了新陈代谢。我们得出结论，通过这种形式的相互作用，神经元可以影响大脑中的肿瘤行为。

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