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首页> 外文期刊>The European Journal of Neuroscience >Differential distribution of KCC2 along the axo-somato-dendritic axis of hippocampal principal cells.
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Differential distribution of KCC2 along the axo-somato-dendritic axis of hippocampal principal cells.

机译：KCC2沿海马主细胞的轴突-树突轴的差异分布。

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摘要

The neuron-specific potassium-chloride cotransporter 2 (KCC2) plays a crucial role in adjusting intracellular Cl(-) concentrations. The lack of KCC2 in the plasma membrane of the axon initial segment (AIS) of pyramidal cells contributes to variable reversal potentials for perisomatic gamma-aminobutyric acid (GABA)(A) receptor-mediated postsynaptic potentials, but the distribution of KCC2 in pyramidal dendrites remains to be established. We applied high-resolution pre-embedding immunolocalization to quantify KCC2 concentrations along dendritic, somatic and axonal regions of rat hippocampal principal cells. Confirming our results on neocortical pyramidal cells, membranes of AIS of CA1 pyramidal cells and dentate granule cells contained 6.4 +/- 11.9% and 6.6 +/- 14.1% of somatic KCC2 concentrations, respectively. Concentrations of KCC2 in basal dendritic shafts of stratum (str.) oriens were similar to somatic levels (109.2 +/- 48.8%). Along apical dendritic shafts of CA1 pyramidal cells, the concentration of KCC2 showed a complex profile: normalized to somatic levels, the density of KCC2 was 124.5 +/- 15.7%, 79 +/- 12.4% and 98.2 +/- 33.5% in the proximal and distal part of str. radiatum and in str. lacunosum moleculare, respectively. Dendritic spines of CA1 receiving excitatory inputs contained 39.9 +/- 8.5% of KCC2 concentration measured in shafts of the same dendritic segments targeted by GABAergic inputs. Dendrites of dentate granule cells showed higher KCC2 concentration compared with the soma (148.9 +/- 54%), but no concentration gradient was detected between proximal and distal dendrites. In conclusion, the density of KCC2 in hippocampal principal cells increases along the axo-somato-dendritic axis with cell type-specific distribution profiles within the dendritic tree.
机译：神经元特异性氯化钾共转运蛋白2（KCC2）在调节细胞内Cl（-）浓度中起关键作用。锥体细胞轴突起始节（AIS）质膜中缺乏KCC2导致过适的γ-氨基丁酸（GABA）（A）受体介导的突触后电位的可变逆转电位，但KCC2在锥体树突中的分布有待确定。我们应用高分辨率的嵌入前免疫定位，以量化大鼠海马主要细胞的树突状，体细胞和轴突区域的KCC2浓度。证实我们在新皮层锥体细胞上的结果，CA1锥体细胞和齿状颗粒细胞的AIS膜分别包含体细胞KCC2浓度的6.4 +/- 11.9％和6.6 +/- 14.1％。 Oriens基层的树突状干中的KCC2浓度与体细胞水平相似（109.2 +/- 48.8％）。沿着CA1锥体细胞的顶端树突轴，KCC2的浓度显示出复杂的分布：归一化为体细胞水平，KCC2的密度为124.5 +/- 15.7％，79 +/- 12.4％和98.2 +/- 33.5％。 str的近端和远端。 radiatum和in。腔虫分子。接受兴奋性输入的CA1的树突棘所含KCC2浓度为39.9 +/- 8.5％，在GABA能量输入所靶向的相同树突节的轴中测得。与体细胞相比，齿状颗粒细胞的树突状细胞显示出较高的KCC2浓度（148.9 +/- 54％），但在近端和远端树突之间未检测到浓度梯度。总之，海马主要细胞中KCC2的密度沿树突状树内具有细胞类型特异性分布特征的轴突-躯体-树突轴增加。

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