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首页> 外文期刊>The European Journal of Neuroscience >Gene expression analysis in the parvalbumin-immunoreactive PV1 nucleus of the mouse lateral hypothalamus.
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Gene expression analysis in the parvalbumin-immunoreactive PV1 nucleus of the mouse lateral hypothalamus.

机译：小鼠下丘脑小白蛋白免疫反应性PV1核中的基因表达分析。

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A solitary, elongated cluster of parvalbumin-immunoreactive neurons has been previously observed in the rodent ventrolateral hypothalamus. However, the function of this so-called PV1 nucleus is unknown. In this article, we report the results of an unbiased, broad and in-depth molecular characterization of this small, compact group of neurons. The Allen Brain Atlas database of in situ hybridization was screened in order to identify genes expressed in the PV1-nucleus-containing area of the hypothalamus, and those that might be co-expressed with parvalbumin. Although GABA is the principal neurotransmitter in parvalbumin-expressing cells in various other brain areas, we found that PV1 neurons express the vesicular glutamate transporter (VGlut) VGlut2-encoding gene Slc17a6 and are negative for the glutamic acid decarboxylase 1 (GAD1) gene. These cells also express the mRNA for the neuropeptides Adcyap1 and possibly Nxph4, express several types of potassium and sodium channels, are under the control of the neurotransmitter acetylcholine, bear receptors for the glial-derived neurotrophic factor, and produce an extracellular matrix rich in osteopontin. The PV1 nucleus is thus composed of glutamatergic nerve cells, expressing some typical markers of long-axon, projecting neurons (e.g. VGlut2), but also co-expressing genes typical of short-axon GABA neurons (e.g. a variety of potassium channels). Hence, neurons of the PV1 nucleus combine physiological characteristics of interneurons with those of projection neurons.
机译：先前在啮齿动物腹外侧下丘脑中观察到一个孤儿，细长的小白蛋白免疫反应性神经元簇。但是，这个所谓的PV1核的功能尚不清楚。在本文中，我们报告了这种小型而紧凑的神经元组的无偏，广泛而深入的分子表征结果。筛选了原位杂交的Allen Brain Atlas数据库，以鉴定在下丘脑包含PV1核的区域表达的基因，以及可能与小白蛋白共表达的基因。尽管GABA是其他大脑各个区域表达小白蛋白的细胞中的主要神经递质，但我们发现PV1神经元表达囊泡谷氨酸转运蛋白（VGlut）VGlut2编码基因Slc17a6，并且对谷氨酸脱羧酶1（GAD1）基因呈阴性。这些细胞还表达神经肽Adcyap1和可能的Nxph4的mRNA，表达几种类型的钾和钠通道，在神经递质乙酰胆碱的控制下，承担神经胶质源性神经营养因子的受体，并产生富含骨桥蛋白的细胞外基质。因此，PV1核由谷氨酸能神经细胞组成，它们表达长轴突投射神经元的一些典型标记（例如VGlut2），还共同表达短轴突GABA神经元的典型基因（例如各种钾通道）。因此，PV1核的神经元将中间神经元的生理特性与投射神经元的生理特性结合在一起。

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