...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Corticotropin-releasing factor type-2 receptor and corticotropin-releasing factor-binding protein coexist in rat ventral tegmental area nerve terminals originated in the lateral hypothalamic area
【24h】

Corticotropin-releasing factor type-2 receptor and corticotropin-releasing factor-binding protein coexist in rat ventral tegmental area nerve terminals originated in the lateral hypothalamic area

机译：促肾上腺皮质激素释放因子2型受体和促肾上腺皮质激素释放因子结合蛋白在下丘脑外侧区的大鼠腹侧被盖区神经末梢共存

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is significant functional evidence showing that corticotropin-releasing factor type-2 receptor (CRF2R) and corticotropin-releasing factor-binding protein (CRF-BP) regulate glutamatergic synapses onto ventral tegmental area (VTA) dopaminergic neurons. It has been shown that CRF requires CRF-BP to potentiate N-methyl-D-aspartate receptors in dopaminergic neurons through CRF2R, and that increases glutamate release in cocaine-treated rats through the activation of CRF2R only by agonists with high affinity to CRF-BP. Furthermore, this CRF-mediated increase in VTA glutamate is responsible for stress-induced relapse to cocaine-seeking behaviour. However, there is a lack of anatomical evidence to explain the mechanisms of CRF actions in VTA. Thus, it was studied whether CRF2R and CRF-BP are expressed in VTA nerve terminals, using a synaptosomal preparation devoid of postsynaptic elements. The current results show that both proteins are co-expressed in glutamatergic and c-aminobutyric acid (GABA) ergic VTA synaptosomes. A main glutamatergic input to the VTA that has been associated to addictive behaviour is originated in the lateral hypothalamic area (LHA). Thus, this study was focused in the LHA-VTA input using orexin as a marker of this input. The results show that CRF2R and CRF-BP mRNA and protein are expressed in the LHA, and that both proteins are present in orexin-positive VTA synaptosomes. The results showing that CRF2R and CRF-BP are expressed in the LHA-VTA input give anatomical support to suggest that this input plays a role in stress-induced relapse to cocaine-seeking behaviour.
机译：有重要的功能证据表明，促肾上腺皮质激素释放因子2型受体（CRF2R）和促肾上腺皮质激素释放因子结合蛋白（CRF-BP）调节谷氨酸能突触到达腹侧被盖区（VTA）多巴胺能神经元。研究表明，CRF要求CRF-BP通过CRF2R增强多巴胺能神经元中的N-甲基-D-天冬氨酸受体，并且仅通过对CRF-R具有高亲和力的激动剂激活CRF2R来增加可卡因治疗大鼠的谷氨酸释放BP。此外，这种CRF介导的VTA谷氨酸含量增加是应激诱导的可卡因寻求行为复发的原因。但是，缺乏解剖学证据来解释VTA中CRF动作的机制。因此，研究了使用无突触后元件的突触体制剂在VTA神经末梢中是否表达CRF2R和CRF-BP。目前的结果表明，这两种蛋白在谷氨酸能和c-氨基丁酸（GABA）的VTA突触小体中共表达。与成瘾行为有关的VTA的主要谷氨酸能输入起源于下丘脑外侧区域（LHA）。因此，本研究集中在使用orexin作为该输入标记的LHA-VTA输入中。结果表明，CRF2R和CRF-BP mRNA和蛋白在LHA中表达，并且两种蛋白都存在于orexin阳性VTA突触小体中。结果表明，在LHA-VTA输入中表达了CRF2R和CRF-BP提供了解剖学支持，表明该输入在应激诱导的可卡因寻求行为复发中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号