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首页> 外文期刊>The European Journal of Neuroscience >Independent receptors for diadenosine pentaphosphate and ATP in rat midbrain single synaptic terminals.
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Independent receptors for diadenosine pentaphosphate and ATP in rat midbrain single synaptic terminals.

机译：大鼠中脑单突触末端的五磷酸腺苷和ATP的独立受体。

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摘要

Diadenosine pentaphosphate (Ap5A) and adenosine 5'-triphosphate (ATP) stimulate a intrasynaptosomal calcium concentration [Ca(2+)](i) increase via specific purinergic receptors in rat midbrain synaptosomes, although nothing is known about their distribution in presynaptic terminals. A microfluorimetric technique to measure [Ca(2+)](i) increase using the dye FURA-2AM, has permitted study of the presence of dinucleotide and P2X receptors in independent isolated synaptic terminals. Our results demonstrate the existence of three populations of synaptosomes: one with dinucleotide receptors (12%), another with P2X receptors (20%) and a third with both (14%). It has been possible to demonstrate that the activation of these receptors occurs only in the presence of extracellular Ca(2+) and that it is also coupled with voltage-dependent Ca(2+) channels. Finally 54% of the synaptosomes that responded to K(+) did not present any calcium increase mediated by the nucleotides used. In summary, ATP and dinucleotides exhibit specific ionotropic receptors that can coexist or not on the same synaptic terminal.
机译：五磷酸腺苷（Ap5A）和5'-三磷酸腺苷（ATP）刺激大鼠中脑突触体中特定的嘌呤能受体引起的突触体内钙浓度[Ca（2 +）]（i）升高，尽管关于它们在突触前末端的分布尚无定论。一种微荧光技术来测量[Ca（2 +）]（i）使用染料FURA-2AM的增加，已经允许研究独立的突触末端中二核苷酸和P2X受体的存在。我们的研究结果表明存在三个突触小体群体：一个带有二核苷酸受体（12％），另一个带有P2X受体（20％），第三个都带有（14％）。可能已经证明这些受体的激活仅在细胞外Ca（2+）的存在下发生，并且还与电压依赖性Ca（2+）通道耦合。最终，对K（+）有反应的突触小体中有54％的核苷酸没有显示出钙的增加。总之，ATP和二核苷酸显示特定的离子受体，可以共存或不在同一突触末端。

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