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首页> 外文期刊>The European Journal of Neuroscience >Multiple-site optical recording reveals embryonic organization of synaptic networks in the chick spinal cord.
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Multiple-site optical recording reveals embryonic organization of synaptic networks in the chick spinal cord.

机译：多位置光学记录揭示了雏鸡脊髓中突触网络的胚胎组织。

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摘要

We examined embryonic expression of postsynaptic potentials in stages 26-31 (E5 to E7) chick spinal cord slices. Slow optical signals related to the postsynaptic potentials which were evoked by electrical stimulation of afferent fibers were identified in the dorsal grey matter and the ventral motoneuronal area. In cervical spinal cord (C13) preparations, the dorsal slow signal appeared from stage 28 (E6), whilst the ventral slow signal was recognized from stage 29. At stages 26 and 27 (E5), no slow signal was observed in either the dorsal or ventral regions. On the other hand, in lumbosacral spinal cord (LS5) preparations, the dorsal, as well as ventral, slow signals appeared from stage 29; at stage 28 no slow signal was detected in the dorsal or ventral regions. These results suggest that there are differences in the ontogenetic expression of synaptic functions between the dorsal and ventral regions, and between the cervical and lumbosacral spinal cords. In embryos older than stage 29, removal of Mg2+ from the bathing solution markedly enhanced the amplitude and incidence of the ventral slow signal. In addition, in C13 preparations at stage 28, removal of Mg2+ elicited small slow signals in the ventral region in which no synaptic response was evoked in normal Ringer's solution. The slow signals induced in the Mg2+-free solution were blocked by 2-amino-5-phosphonovaleric acid (APV), showing that they are attributable to N-methyl- D-aspartate (NMDA) receptors. These results suggest that functional synaptic connections via polysynaptic pathways are already generated on motoneurons, but are suppressed by a Mg2+ block on the NMDA receptors at developmental stages when synaptic transmission from the primary afferents to the dorsal interneurons is initially expressed in the dorsal region.
机译：我们检查了阶段26-31（E5至E7）小鸡脊髓切片中突触后电位的胚胎表达。在背灰质和腹膜上皮区发现了与突触后电位有关的慢光信号，这些信号是由传入纤维的电刺激引起的。在颈脊髓（C13）制剂中，背侧慢信号出现在第28阶段（E6），而腹侧慢信号出现在第29阶段。在第26和27阶段（E5），在任一背侧均未观察到慢信号或腹侧区域。另一方面，在腰s部脊髓（LS5）制剂中，从第29阶段开始出现背侧和腹侧的缓慢信号;在阶段28，在背侧或腹侧区域未检测到慢信号。这些结果表明，在背侧和腹侧区域以及子宫颈和腰s脊髓之间的突触功能的本体表达中存在差异。在年龄超过29岁的胚胎中，从沐浴液中去除Mg2 +明显增强了腹侧慢信号的幅度和发生率。此外，在第28阶段的C13制剂中，Mg2 +的去除在腹侧区域引起小的缓慢信号，在正常的林格氏液中未引起突触反应。在无Mg2 +溶液中诱导的慢信号被2-氨基-5-膦酰戊酸（APV）阻断，表明它们可归因于N-甲基-D-天冬氨酸（NMDA）受体。这些结果表明，通过多突触途径的功能性突触连接已经在运动神经元上产生，但是在发育阶段，当最初从初级传入神经向背侧中神经元的突触传递最初在背侧区域表达时，NMDA受体上的Mg2 +阻滞会抑制这种连接。

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