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首页> 外文期刊>The European Journal of Neuroscience >Neuronal activation and corticotropin-releasing hormone expression in the brain of obese (fa/fa) and lean (fa/?) Zucker rats in response to refeeding.
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Neuronal activation and corticotropin-releasing hormone expression in the brain of obese (fa/fa) and lean (fa/?) Zucker rats in response to refeeding.

机译：肥胖（fa / fa）和瘦（fa /？）Zucker大鼠的大脑神经元活化和促肾上腺皮质激素释放激素表达，以应对其重新喂养。

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摘要

The present study was conducted to investigate the pattern of neuronal activation and corticotropin-releasing hormone (CRH) expression in fed, food deprived and refed lean (Fa/?) and obese (fa/fa) Zucker rats. The pattern of neuronal activation was studied by measuring the expression of the immediate-early gene c-fos. Expression of c-fos and CRH mRNA was determined by in situ hybridization histochemistry. In both lean and obese rats, one hour of refeeding led to a transient increase in c-fos mRNA levels which was detected in the paraventricular hypothalamic nucleus (PVH), the dorsomedial hypothalamic nucleus, the supraoptic nucleus, the paraventricular thalamic nucleus, the central nucleus of amygdala (CeA), the lateral and medial parabrachial nuclei, the nucleus of the solitary tract, and the area postrema. In addition, refeeding led to strong activation of the arginine-vasopressin neurons located in the magnocellular part of the PVH. Following 24 h of food deprivation, CRH expression in the parvocellular division of the PVH was significantly higher in obese rats compared to lean animals. During refeeding, PVH CRH mRNA levels in obese rats decreased to reach control values. The decrease in CRH expression in obese rats was accompanied by the alleviation of the hypercorticosteronemia that characterized obese Zucker rats. CRH mRNA levels in the central nucleus of the amygdala were significantly higher in lean rats than in obese animals, when the rats were fed ad libitum During food deprivation, CeA CRH mRNA levels decreased in lean rats and gradually returned to predeprivation values during refeeding. In refed obese rats, CeA levels of CRH mRNA were higher than those of ad libitum fed or food-deprived obese mutants. In the perifornical region of the lateral hypothalamic area (LHA), the expression of CRH mRNA rose significantly in response to refeeding in lean rats, but not in obese animals. Following the first hour of refeeding, the number of neurons expressing CRH mRNA in the LHA in lean rats almost doubled. The present results demonstrate that refeeding has a stimulating effect in obese Zucker rats in a pattern of activation similar to that seen in lean Fa/? rats. They also demonstrate differences in CRH expression between Fa/? and fa/fa rats after refeeding. The most apparent of these differences was seen in the lateral hypothalamus in which refeeding failed to up-regulate CRH expression in obese rats.
机译：进行本研究以研究在进食，缺乏食物和拒绝瘦肉（Fa /？）和肥胖（fa / fa）的Zucker大鼠中神经元活化和促肾上腺皮质激素释放激素（CRH）表达的模式。通过测量立即早期基因c-fos的表达来研究神经元激活的模式。通过原位杂交组织化学测定c-fos和CRH mRNA的表达。在瘦和肥胖的大鼠中，再喂养一小时都会导致c-fos mRNA水平短暂升高，这在室下丘脑下核（PVH），背丘脑下丘脑核，视上核，丘脑室旁核，中央中央被检测到杏仁核（CeA），外侧臂和内侧臂旁核，孤立道核和后区域。此外，补饲导致位于PVH巨细胞部分的精氨酸-加压素神经元的强烈激活。禁食24小时后，肥胖大鼠的PVH小细胞分裂中CRH表达明显高于瘦肉动物。在重新喂养期间，肥胖大鼠的PVH CRH mRNA水平降低至控制值。肥胖大鼠中CRH表达的减少伴随着肥胖的Zucker大鼠的皮质激素过多血症的减轻。瘦大鼠的杏仁核中央核中的CRH mRNA水平显着高于肥胖动物，当大鼠自由进食时，在食物匮乏时，瘦大鼠中的CeA CRH mRNA水平下降，并在再喂养期间逐渐恢复到剥夺前的水平。在肥胖的肥胖大鼠中，CeA CRH mRNA水平高于随意喂养或缺乏食物的肥胖突变体。在下丘脑外侧区域（LHA）的前穹peri区域，CRH mRNA的表达在瘦大鼠中响应于进食而显着上升，但在肥胖动物中则没有。再喂食的第一小时后，瘦鼠LHA中表达CRH mRNA的神经元数量几乎翻了一番。目前的结果表明，再喂养对肥胖的Zucker大鼠具有刺激作用，其激活方式类似于在贫Fa /α中所见。大鼠。他们还证明了Fa /α之间CRH表达的差异。再喂食后的fa和fa / fa大鼠。这些差异中最明显的是在下丘脑外侧，在肥胖的大鼠中，进食未能上调CRH表达。

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