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首页> 外文期刊>The European Journal of Neuroscience >Differential induction and localization of mPer1 and mPer2 during advancing and delaying phase shifts.
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Differential induction and localization of mPer1 and mPer2 during advancing and delaying phase shifts.

机译：在推进和延迟相移过程中对mPer1和mPer2的差分感应和定位。

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The mechanism whereby brief light exposure resets the mammalian circadian clock in a phase dependent manner is not known, but is thought to involve Per gene expression. At the behavioural level, a light pulse produces phase delays in early subjective night, phase advances in late subjective night, and no phase shifts in mid-subjective night or subjective day. To understand the relationship between Per gene activity and behavioural phase shifts, we examined light-induced mPer1 and mPer2 expression in the suprachiasmatic nucleus (SCN) of the mouse, in the subjective night, with a view to understanding SCN heterogeneity. In the VIP-containing region of the SCN (termed 'core'), light-induced mPer1 expression occurs at all times of the subjective night, while mPer2 induction is seen only in early subjective night. In the remaining regions of the SCN (termed 'shell'), a phase delaying light pulse produces no mPer1 but significant mPer2 expression, while a phase advancing light pulse produces no mPer2 but substantial mPer1 induction. Moreover, following a light pulse during mid-subjective night, neither mPer1 nor mPer2 are induced in the shell. The results reveal that behavioural phase shifts occur only when light-induced Per gene expression spreads from the core to the shell SCN, with mPer1 expression in shell corresponding to phase advances, and mPer2 corresponding to phase delays. The results indicate that the time course and the localization of light-induced Per gene expression in SCN reveals important aspects of intra-SCN communication.
机译：短时曝光以相位依赖方式重置哺乳动物生物钟的机制尚不清楚，但据认为涉及Per基因表达。在行为水平上，光脉冲在主观夜晚的早期产生相位延迟，在主观夜晚的晚期产生相位延迟，而在主观夜晚或主观白天则没有相移。为了了解Per基因活性与行为相移之间的关系，我们在主观的夜晚检查了小鼠上眼交叉核（SCN）中光诱导的mPer1和mPer2表达，以了解SCN的异质性。在SCN的VIP区域（称为“核心”），光诱导的mPer1表达始终在主观夜晚出现，而mPer2诱导仅在主观夜晚出现。在SCN的其余区域（称为“壳”）中，延迟相位的光脉冲不产生mPer1，而产生明显的mPer2表达，而相位前进的光脉冲则不产生mPer2，而是产生实质性的mPer1感应。此外，在主观夜晚的夜晚发出光脉冲之后，在壳中均未诱导出mPer1和mPer2。结果表明，仅当光诱导的Per基因表达从核向壳SCN扩散时，行为相移才会发生，壳中的mPer1表达与相提前相对应，而mPer2的表达与相延迟相对应。结果表明，时间过程和光诱导的Per基因在SCN中的表达定位揭示了SCN内部通讯的重要方面。

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