...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Control of kinetic properties of GluR2 flop AMPA-type channels: impact of R/G nuclear editing.
【24h】

Control of kinetic properties of GluR2 flop AMPA-type channels: impact of R/G nuclear editing.

机译：控制GluR2触发器AMPA型通道的动力学特性：R / G核编辑的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The GluR2 flop subunit of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors greatly determines calcium permeability and kinetic properties of heteromeric AMPA subunit assemblies. Post-transcriptional editing of this subunit at the Q/R/N site controls calcium permeability whereas editing at the R/G site is involved in the regulation of biophysical properties. We used patch-clamp techniques with ultrafast solution exchange to examine the kinetics of recombinant human homomeric GluR2 flop channels transiently expressed in HEK293 cells [edited at the R/G site and Q/R/N site (GR), and unedited (RN) and edited (GN) at the R/G site both with asparagine (N) at the Q/R/N site]. The time constant of desensitization after application of 10 mm glutamate was 1.38 +/- 0.05 ms (n = 10), 5.53 +/- 0.57 ms (n = 7) and 1.33 +/- 0.06 ms (n = 12) for the GluR2 flop GR, RN and GN channels, respectively. The time constant of resensitization was 75 ms for the GluR2 flop RN and 30 ms for the GN channels. The dose-dependence of the peak current amplitude, kinetics of activation and deactivation, and peak open probability did not differ between RN and GN channels. The study shows that desensitization and resensitization kinetics of homomeric GluR2 flop channels are controlled by a single amino acid exchange (glycine by arginine) at the R/G site. Quantitative analysis by computer simulation using a circular kinetic scheme allows the prediction of the main experimental results.
机译：α-氨基-3-羟基-5-甲基异恶唑-4-丙酸（AMPA）型谷氨酸受体的GluR2 flop亚基极大地决定了钙离子渗透性和杂聚AMPA亚基组件的动力学性质。在Q / R / N位点进行此亚基的转录后编辑可控制钙的通透性，而在R / G位点进行的编辑涉及生物物理特性的调节。我们使用膜片钳技术与超快速溶液交换来检查在HEK293细胞中瞬时表达的重组人同源GluR2翻转通道的动力学[在R / G位点和Q / R / N位点（GR）编辑，未编辑（RN）并在Q / R / N位置用天冬酰胺（N）在R / G位置进行编辑（GN）]。对于GluR2，应用10毫米谷氨酸盐后脱敏的时间常数为1.38 +/- 0.05毫秒（n = 10），5.53 +/- 0.57毫秒（n = 7）和1.33 +/- 0.06毫秒（n = 12）。触发器GR，RN和GN通道。对于GluR2触发器RN，重新敏感的时间常数为75毫秒，对于GN通道，为30毫秒。 RN和GN通道之间的峰值电流幅度，激活和失活动力学以及峰值打开概率的剂量依赖性无差异。研究表明，同质GluR2 flop通道的脱敏和再敏化动力学受R / G位点的单个氨基酸交换（甘氨酸精氨酸）控制。使用循环动力学方案通过计算机模拟进行的定量分析可以预测主要的实验结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号