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首页> 外文期刊>The European Journal of Neuroscience >Glucocorticoids alter calcium conductances and calcium channel subunit expression in basolateral amygdala neurons.
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Glucocorticoids alter calcium conductances and calcium channel subunit expression in basolateral amygdala neurons.

机译：糖皮质激素改变基底外侧杏仁核神经元中的钙电导和钙通道亚单位表达。

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摘要

Glucocorticoid hormones, which are released in high amounts after stress, enter the brain where they bind to intracellular receptors that are abundant in limbic areas, in particular the hippocampus and amygdala nuclei. Behavioural studies indicate that glucocorticoids modulate learning and memory processes via receptors in the hippocampus and amygdala. So far, the effects of glucocorticoids on amygdala neurons have not been investigated at the cellular and molecular level. We report here that in vitro application of glucocorticoids for 20 min increases 1-4 h later the amplitude of sustained, high-voltage-activated calcium currents in principal neurons of the basolateral amygdala. In contrast, the transient, low-voltage-activated currents were decreased. We examined whether these functional changes in calcium conductance were accompanied by transcriptional regulation of calcium channel subunits. Analysis of the RNA - collected after recording and then linearly amplified - revealed that glucocorticoid-mediated increases in sustained calcium currents are associated with a parallel shift in the relative expression of the alpha1 subunit constituting the pore of the sustained, high-voltage-activated (L-type) calcium channel. These data indicate that glucocorticoids, probably by selectively targeting genes encoding calcium channel subunits, largely alter the calcium influx into basolateral amygdala neurons. These actions could modify amygdala network function and thus contribute to the behavioural effects exerted by the stress hormones via the basolateral amygdala.
机译：应激后大量释放的糖皮质激素进入大脑，并与边缘区丰富的细胞内受体结合，尤其是海马和杏仁核。行为研究表明，糖皮质激素通过海马和杏仁核中的受体调节学习和记忆过程。迄今为止，尚未在细胞和分子水平上研究糖皮质激素对杏仁核神经元的作用。我们在这里报告说，糖皮质激素的体外应用20分钟会增加1-4小时，此后在基底外侧杏仁核的主要神经元中持续，高压激活的钙电流的幅度增加。相反，瞬态的低压激活电流降低了。我们检查了钙电导的这些功能性变化是否伴随着钙通道亚基的转录调控。对RNA的分析-记录后收集，然后进行线性扩增-发现，糖皮质激素介导的持续钙电流增加与构成持续的，高电压激活的（ L型）钙通道。这些数据表明，糖皮质激素可能通过选择性地靶向编码钙通道亚基的基因，大大改变了钙流入基底外侧杏仁核神经元的数量。这些动作可能会改变杏仁核的网络功能，从而有助于压力激素通过基底外侧杏仁核施加的行为效应。

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