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首页> 外文期刊>The European Journal of Neuroscience >TRKB signalling controls the expression of N-methyl-d-aspartate receptors in the visual cortex.
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TRKB signalling controls the expression of N-methyl-d-aspartate receptors in the visual cortex.

机译：TRKB信号控制视觉皮层中N-甲基-d-天冬氨酸受体的表达。

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NMDA receptors (NMDARs) are multimeric proteins, the biological and functional characteristics of which depend on differential subunit assembly during postnatal development. In the present paper, we investigated whether the expression of NMDAR subunits NR1, NR2A, NR2B is influenced by neurotrophins in rat visual cortex. We used a soluble form of the TrkB receptor engineered as an immunoadhesin (TrkB-IgG) in order to block TrkB ligands. TrkB-IgG was released through a cannula implanted in the occipital pole and connected to a mini-osmotic pump. TrkB-IgG was continuously released from postnatal day 20-21 (P20-21) to P36-37. In a different group of animals used as controls, osmotic pumps were filled with saline. Different antibodies were used to stain neurons expressing NR1, NR2A and NR2B. We counted the number of neurons stained for NR2A and NR2B subunits and expressed this as percentage with respect to the total number of cresyl-violet stained neurons in each cortical layer. In the visual cortex of TrkB-IgG-treated rats, the percentage of neurons expressing NR2A was significantly increased in all cortical layers. Concerning the NR2B subunit, the percentage of stained neurons was not significantly different between TrkB-IgG-treated and control rats. The staining level for both NR2A and NR2B, but not NR1, was reduced in all cortical layers in TrkB-IgG-treated animals. In agreement with this result, the endogenous levels of NR2A and NR2B subunits were reduced in TrkB-IgG-treated animals as shown by Western blotting. Thus, TrkB signalling controls the cellular expression of NMDAR subunits in visual cortical neurons during postnatal development.
机译：NMDA受体（NMDAR）是多聚体蛋白，其生物学和功能特性取决于出生后发育过程中差异化的亚基组装。在本文中，我们调查了NMDAR亚基NR1，NR2A，NR2B的表达是否受到大鼠视皮层神经营养因子的影响。我们使用了可溶形式的TrkB受体，将其设计为免疫粘附素（TrkB-IgG），以阻断TrkB配体。 TrkB-IgG通过植入枕骨的插管释放，并连接到微型渗透泵。 TrkB-IgG从出生后20-21天（P20-21）连续释放到P36-37。在用作对照的另一组动物中，渗透泵中充满了盐水。使用不同的抗体对表达NR1，NR2A和NR2B的神经元染色。我们计算了NR2A和NR2B亚基染色的神经元的数量，并将其表示为相对于每个皮层中甲酚紫染色的神经元总数的百分比。在经TrkB-IgG处理的大鼠的视皮层中，表达NR2A的神经元的百分比在所有皮层中均显着增加。关于NR2B亚基，TrkB-IgG治疗组和对照组大鼠的染色神经元百分比没有显着差异。在TrkB-IgG处理的动物的所有皮质层中，NR2A和NR2B的染色水平均降低，但NR1均未降低。与该结果一致，如Western印迹所示，在经TrkB-IgG处理的动物中，NR2A和NR2B亚单位的内源性水平降低。因此，TrkB信号传导控制出生后发育过程中视觉皮层神经元中NMDAR亚基的细胞表达。

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