...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Distinct expression patterns of inwardly rectifying potassium currents in developing cerebellar granule cells of the hemispheres and the vermis
【24h】

Distinct expression patterns of inwardly rectifying potassium currents in developing cerebellar granule cells of the hemispheres and the vermis

机译：发育中的半球和ver小脑颗粒细胞内向整流钾电流的不同表达模式

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

G-protein-coupled inwardly rectifying potassium (GIRK) channels play a crucial role during the migration and maturation of cerebellar granule cells (GCs) in the vermis. In the cerebellar hemispheres, however, only minor effects on the development of GCs are observed in mice with GIRK channel impairment. This regional difference may reflect distinct ontogenetic expression patterns of GIRK channels. Therefore, inwardly rectifying responses in mice were characterized at different stages of development in the vermis and the hemispheres. In the vermis, GCs in the premigratory zone (PMZ) at P7-P15 exhibit GIRK current but not constitutive inwardly rectifying potassium (CIRK) current, and are relatively depolarized at rest. In contrast, premigratory GCs in the hemispheres express only CIRK channels, which accounts for their more hyperpolarized resting membrane potential. Furthermore, the pattern of voltage-dependent inward currents in the PMZ GCs of cerebellar hemispheres is consistent with a more mature stage of development than the corresponding GCs in the vermis, resulting in robust firing properties mediated by sodium channels. Later in development (P21-P22), CIRK current is then observed in the majority of vermis GCs. This developmental pattern, revealed by electrophysiological recordings, was confirmed by immunohistological experiments that showed greater reactivity for GIRK2 in the PMZ of the vermis than in the hemispheres during development (P7-P15). These findings suggest that regional differences in development are responsible for the differential expression of inwardly rectifying potassium channels in the vermis and in the hemispheres.
机译：G蛋白偶联的内向整流钾（GIRK）通道在the小脑颗粒细胞（GCs）的迁移和成熟过程中起着至关重要的作用。然而，在小脑半球中，在GIRK通道受损的小鼠中，仅观察到了对GC发育的微小影响。这种区域差异可能反映了GIRK通道不同的本体表达模式。因此，小鼠的向内纠正反应的特征是在the和半球发育的不同阶段。在the骨中，P7-P15处的迁移前区（PMZ）中的GC表现为GIRK电流，但没有本构性向内整流钾（CIRK）电流，并且在静止时相对去极化。相比之下，半球中的迁移前GC仅表达CIRK通道，这解释了它们的超极化静息膜电位。此外，小脑半球PMZ GC中与电压有关的内向电流模式比the骨中相应的GC更成熟，这与钠通道介导的稳固发射特性相一致。在开发的后期（P21-P22），然后在大多数Vermis GC中观察到CIRK电流。通过电生理记录揭示的这种发育模式已通过免疫组织学实验证实，该实验显示，在发育过程中，mis的PMZ中对GIRK2的反应性高于半球中的GIRK2（P7-P15）。这些发现表明，发育中的区域差异是导致the和半球内向整流钾通道差异表达的原因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号