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首页> 外文期刊>The European Journal of Neuroscience >Brain extracellular glucose assessed by voltammetry throughout the rat sleep-wake cycle.
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Brain extracellular glucose assessed by voltammetry throughout the rat sleep-wake cycle.

机译：在整个大鼠睡眠-觉醒周期中，通过伏安法评估大脑的细胞外葡萄糖。

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In the present study, cortical extracellular levels of glucose were monitored for the first time throughout the sleep-wake states of the freely moving rat. For this purpose, polygraphic recordings (electroencephalogram of the fronto-occipital cortices and electromyogram of the neck muscles) were achieved in combination with differential normal pulse voltammetry (DNPV) using a specific glucose sensor. Data obtained reveal that the basal extracellular glucose concentration in the conscious rat is 0.59 +/- 0.3 m M while under chloral hydrate anaesthesia (0.4 g/kg, i.p.) it increases up to 180% of its basal concentration. Regarding the sleep-wake cycle, the existence of spontaneous significant variations in the mean glucose level during slow-wave sleep (SWS = +13%) and paradoxical sleep (PS = -11%) compared with the waking state (100%) is also reported. It is to be noticed that during long periods of active waking, glucose level tends towards a decrease that becomes significant after 15 min (active waking = -32%). On the contrary, during long episodes of slow-wave sleep, it tends towards an increase which becomes significant after 12 min (SWS = +28%). It is suggested that voltammetric techniques using enzymatic biosensors are useful tools allowing direct glucose measurements in the freely moving animal. On the whole, paradoxical sleep is pointed out as a state highly dependent on the availability of energy and slow-wave sleep as a period of energy saving.
机译：在本研究中，在自由活动大鼠的整个睡眠-觉醒状态下，首次监测了皮质的细胞外葡萄糖水平。为此，结合使用特定的葡萄糖传感器的差分正常脉搏伏安法（DNPV），可以实现多项记录记录（额枕皮层脑电图和颈部肌肉肌电图）。获得的数据表明，在清醒大鼠中基础细胞外葡萄糖浓度为0.59 +/- 0.3 m M，而在水合氯醛麻醉下（0.4 g / kg，i.p.），其升高至其基础浓度的180％。关于睡眠-觉醒周期，与清醒状态（100％）相比，慢波睡眠（SWS = + 13％）和自相矛盾的睡眠（PS = -11％）中平均葡萄糖水平的自发显着变化是也有报道。要注意的是，在长时间的主动苏醒期间，葡萄糖水平趋于下降，在15分钟后变得显着（主动苏醒= -32％）。相反，在长时间的慢波睡眠中，它倾向于增加，在12分钟后变得明显（SWS = + 28％）。建议使用酶生物传感器的伏安技术是有用的工具，可以在自由运动的动物中直接测量葡萄糖。总体上，矛盾的睡眠被认为是高度依赖于能量的可获得性的状态，而慢波睡眠是节能的时期。

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