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首页> 外文期刊>The European Journal of Neuroscience >Evolution of changes in neuronal activity in the subthalamic nucleus of rats with unilateral lesion of the substantia nigra assessed by metabolic and electrophysiological measurements.
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Evolution of changes in neuronal activity in the subthalamic nucleus of rats with unilateral lesion of the substantia nigra assessed by metabolic and electrophysiological measurements.

机译：通过代谢和电生理测量评估黑质单侧病变大鼠丘脑下核神经元活动的变化。

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摘要

Cellular expression of cytochrome oxidase subunit I (COI) mRNA has recently been used as a metabolic marker for neuronal activity to study the functional changes in the subthalamic nucleus (STN) in parkinsonism. The previous experimental studies have been performed when the pathological state was stabilized at a maximal level. In order to determine the evolution of changes in neuronal activity in the STN after nigrostriatal denervation, we analysed by in situ hybridization the cellular expression of COI mRNA in the subthalamic neurons at different times, from 6 h to 14 days, after unilateral intranigral microinjection of 6-hydroxydopamine (6-OHDA) in rats. In parallel, the time-dependent changes of the unit neuronal activity of subthalamic neurons have been recorded. Levels of COI mRNA increased by 41% in subthalamic neurons from 24 h after 6-OHDA intoxication, to 14 days (+26%). Similarly, electrical activity started to increase slightly 24 h after lesion (+20%) and remained significantly higher at 14 days after the lesion (+189%). Changes in neuronal mean discharge rate were associated with changes in the pattern of spiking activity, from a regular firing pattern to an irregular one with a high bursting activity. These results show that: (i) the hyperactivity of the STN represents a very early phenomenon in the physiopathology of parkinsonian syndromes; and (ii) that changes in COI mRNA expression slightly precede changes in electrical neuronal activity.
机译：细胞色素氧化酶亚基I（COI）mRNA的细胞表达最近已被用作神经活动的代谢标记，以研究丘脑下丘脑核（STN）在帕金森病中的功能变化。当病理状态稳定在最大水平时，已经进行了先前的实验研究。为了确定黑质纹状体去神经后STN中神经元活性变化的演变，我们通过原位杂交分析了单侧经鼻背突入后6 h至14天不同时间丘脑下神经元中COI mRNA的细胞表达。大鼠中的6-羟基多巴胺（6-OHDA）。平行地，已经记录了丘脑下神经元的单位神经元活动的时间依赖性变化。从6-OHDA中毒后24小时到14天（+ 26％），丘脑下神经元中COI mRNA的水平增加了41％。同样，电活动在病变后24小时开始略有增加（+ 20％），并在病变后14天仍显着升高（+ 189％）。神经元平均放电速率的变化与尖峰活动模式的变化相关，从规则的发射模式到具有高爆发活动的不规则发射模式。这些结果表明：（i）STN亢进代表帕金森综合征的生理病理学中非常早期的现象; （ii）COI mRNA表达的变化稍早于神经电活动的变化。

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