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首页> 外文期刊>The European Journal of Neuroscience >AMPA receptor activation induces GABA release from neurons migrating tangentially in the intermediate zone of embryonic rat neocortex.
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AMPA receptor activation induces GABA release from neurons migrating tangentially in the intermediate zone of embryonic rat neocortex.

机译：AMPA受体激活可诱导GABA从在大鼠新皮层的中间区域切向迁移的神经元释放。

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摘要

In the intermediate zone of the embryonic rodent neocortex, neurons migrating tangentially from the basal ganglia express both functional amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors and gamma-aminobutyric acid (GABA). To test the hypothesis of GABA release triggered by AMPA receptor activation, we used whole-hemisphere cultures prepared from rat embryos (day 15). We observed a marked decrease in the number of detectable GABA-positive cells in the intermediate zone after exposure to T-AMPA. This effect was blocked by coapplying GYKI 53655, an AMPA receptor antagonist. The decrease in GABA immunolabelling induced by T-AMPA did not require extracellular calcium. In contrast, it was abolished after sodium substitution by choline, or after coapplication of nipecotic acid, a GABA transporter inhibitor. Exposure to high potassium reduced the number of detectable GABA-positive cells. These results are compatible with carrier-mediated GABA release consecutive to sodium influx. GABA released from neurons migrating tangentially in the intermediate zone after AMPA receptor activation may influence neighbouring elements including radially migrating postmitotic neurons, proliferating progenitors and possibly the tangential cells themselves.
机译：在胚胎啮齿动物新皮层的中间区域，从基底神经节切向迁移的神经元同时表达功能性氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体和γ-氨基丁酸（GABA）。为了测试由AMPA受体激活触发的GABA释放的假说，我们使用了从大鼠胚胎制备的全半球培养物（第15天）。我们观察到暴露于T-AMPA后中间区域中可检测的GABA阳性细胞数量显着减少。通过共同使用AMPA受体拮抗剂GYKI 53655阻止了这种作用。 T-AMPA诱导的GABA免疫标记减少不需要细胞外钙。相反，在被胆碱取代钠后，或在共同施用GABA转运蛋白抑制剂ececotic acid后，它被废除了。高钾暴露减少了可检测的GABA阳性细胞的数量。这些结果与钠流入后载体介导的GABA释放是相容的。在AMPA受体激活后，从神经元切向迁移到中间区域的神经元释放的GABA可能影响邻近元件，包括径向迁移的有丝分裂后神经元，增殖祖细胞以及可能的切向细胞本身。

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