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首页> 外文期刊>The European Journal of Neuroscience >Innervation of interneurons immunoreactive for VIP by intrinsically bursting pyramidal cells and fast-spiking interneurons in infragranular layers of juvenile rat neocortex.
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Innervation of interneurons immunoreactive for VIP by intrinsically bursting pyramidal cells and fast-spiking interneurons in infragranular layers of juvenile rat neocortex.

机译：通过内在爆发的幼鼠新皮层下层锥体细胞和快速突触的中间神经对VIP免疫反应的神经内神经的神经支配。

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摘要

Cortical columns contain specific neuronal populations with characteristic sets of connections. This wiring forms the structural basis of dynamic information processing. However, at the single-cell level little is known about specific connectivity patterns. We performed experiments in infragranular layers (V and VI) of rat somatosensory cortex, to clarify further the input patterns of inhibitory interneurons immunoreactive (ir) for vasoactive intestinal polypeptide (VIP). Neurons in acute slices were electrophysiologically characterized using whole-cell recordings and filled with biocytin. This allowed us to determine their firing pattern as regular-spiking, intrinsically bursting and fast-spiking, respectively. Biocytin was revealed histochemically and VIP immunohistochemically. Sections were examined for contacts between the axons of the filled neurons and the VIP-ir targets. Twenty pyramidal cells and five nonpyramidal (inter)neurons were recovered and sufficiently stained for further analysis. Regular-spiking pyramidal cells displayed no axonal boutons in contact with VIP-ir targets. In contrast, intrinsically bursting layer V pyramidal cells showed four putative single contacts with a proximal dendrite of VIP neurons. Fast-spiking interneurons formed contacts with two to six VIP neurons, preferentially at their somata. Single as well as multiple contacts on individual target cells were found. Electron microscopic examinations showed that light-microscopically determined contacts represent sites of synaptic interactions. Our results suggest that, within infragranular local cortical circuits, (i) fast-spiking interneurons are more likely to influence VIP cells than are pyramidal cells and (ii) pyramidal cell input probably needs to be highly convergent to fire VIP target cells.
机译：皮质柱包含具有特征性连接集的特定神经元种群。这种布线构成了动态信息处理的结构基础。但是，在单小区级别，对特定的连接模式知之甚少。我们在大鼠体感皮层的颗粒下层（V和VI）中进行了实验，以进一步阐明对血管活性肠多肽（VIP）的抑制性中间神经元免疫反应（ir）的输入模式。使用全细胞记录对急性切片中的神经元进行电生理学表征，并填充生物素。这使我们可以将它们的触发模式分别确定为常规触发，固有触发和快速触发。生物细胞素通过组织化学和VIP免疫组织化学显示。检查切片以检查填充神经元轴突和VIP-ir靶标之间的接触。回收了二十个锥体细胞和五个非锥体神经元（间），并对它们进行了充分染色以进行进一步分析。定期刺刺的锥体细胞在与VIP-ir靶标接触时未显示轴突突突。相比之下，本质上爆发的V层锥体细胞显示了四个假定的单次接触，与VIP神经元的近端树突相接触。快速爆发的神经元与2至6个VIP神经元形成接触，优先在其躯体处。发现单个靶细胞上的单个以及多个接触。电子显微镜检查表明，光显微镜下确定的接触代表突触相互作用的位点。我们的结果表明，在颗粒下局部皮质回路中，（i）尖峰中枢神经元比锥体细胞更可能影响VIP细胞，并且（ii）锥体细胞输入可能需要高度收敛才能发射VIP目标细胞。

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