...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Molecular-feature domains with posterodorsal-anteroventral polarity in the symmetrical sensory maps of the mouse olfactory bulb: mapping of odourant-induced Zif268 expression.
【24h】

Molecular-feature domains with posterodorsal-anteroventral polarity in the symmetrical sensory maps of the mouse olfactory bulb: mapping of odourant-induced Zif268 expression.

机译：小鼠嗅球对称感官图中具有后气味-前腹侧极性的分子特征域：气味诱导的Zif268表达图谱。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Individual glomeruli in the mammalian olfactory bulb presumably represent a single type of odourant receptor. Thus, the glomerular sheet provides odourant receptor maps at the surface of the olfactory bulb. To understand the basic spatial organization of the olfactory sensory maps, we first compared the spatial distribution of odourant-induced responses measured by the optical imaging of intrinsic signals with that detected immunohistochemically by expressions of Zif268, one of the immediate early gene products in juxtaglomerular cells. In the dorsal surface of the bulb, we detected a clear correlation in the spatial pattern between these responses. In addition, the molecular-feature domains and their polarities (spatial shifts of responses with an increase in carbon chain length) that were defined by the optical imaging method could be also detected by the Zif268 mapping method. We then mapped the Zif268 signals over the entire olfactory bulb using a homologous series of fatty acids and aliphatic alcohols as stimulus odourants. We superimposed the Zif268 signals onto the standard unrolled map with the help of cell adhesion molecule compartments. Each odourant typically elicited two pairs of clusters of dense Zif268 signals. The results showed that molecular-feature domains and their polarities were arranged symmetrically at stereotypical positions in a mirror-image fashion between the lateral and the medial sensory maps. The polarity of each domain was roughly in parallel with the posterodorsal-anteroventral axis that was defined by the cell adhesion molecule compartments. These results suggest that the molecular-feature domain with its fixed polarity is one of the basic structural units in the spatial organization of the odourant receptor maps in the olfactory bulb.
机译：哺乳动物嗅球中的单个肾小球可能代表一种气味受体。因此，肾小球片在嗅球的表面提供了气味受体图。为了了解嗅觉感觉图的基本空间组织，我们首先比较了嗅觉诱发反应的空间分布，该反应是由内在信号的光学成像测量的，与通过免疫组织化学方法检测到的近端肾小球细胞早期基因产物之一Zif268的表达检测到的。 。在灯泡的背面，我们在这些响应之间的空间模式中发现了明显的相关性。此外，还可以通过Zif268映射方法检测由光学成像方法定义的分子特征域及其极性（响应的空间位移，碳链长度增加）。然后，我们使用脂肪酸和脂族醇的同源系列作为刺激性气味，在整个嗅球上绘制Zif268信号。我们借助细胞粘附分子区室将Zif268信号叠加到标准展开图上。每种气味通常会引发两对密集的Zif268信号簇。结果表明，分子特征域及其极性在定型位置对称排列，呈镜像形式，位于外侧和内侧感觉图之间。每个区域的极性与细胞粘附分子区室所定义的后气味-腹膜前轴大致平行。这些结果表明，具有固定极性的分子特征结构域是嗅球中气味受体图谱空间组织的基本结构单位之一。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号