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首页> 外文期刊>The European Journal of Neuroscience >Periwound dopaminergic sprouting is dependent on numbers of wound macrophages.
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Periwound dopaminergic sprouting is dependent on numbers of wound macrophages.

机译：周围多巴胺能发芽取决于伤口巨噬细胞的数量。

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摘要

Injury to many regions of the central nervous system, including the striatum, results in a periwound or 'abortive' sprouting response. In order to directly evaluate whether macrophages play an important role in stimulating periwound sprouting, osteopetrotic (op/op) mice, which when young are deficient in a variety of macrophage subtypes, were given striatal wounds and the degree of dopaminergic sprouting subsequently assessed. Two weeks postinjury, significantly fewer wound macrophages were present in the striata of op/op mice compared with controls (144 +/- 30.1 in op/op mice vs. 416.6 +/- 82.3 in controls, P < 0.005, analysis performed on a section transecting the middle of the wound). Dopamine transporter immunohistochemistry revealed a marked decrease in the intensity of periwound sprouting in the op/op group of animals. Quantification of this effect using [H3]-mazindol autoradiography confirmed that periwound sprouting was reduced significantly in the op/op mice compared with controls (71.4 +/- 21.7 fmol/mg protein in op/op mice vs. 210.7 +/- 27.1 fmol/mg protein in controls, P < 0.0005). In the two groups of animals the magnitude of the sprouting response in individuals was closely correlated with the number of wound macrophages (R = 0.83, R2 = 0.69). Our findings provide strong support for the crucial involvement of macrophages in inducing dopaminergic sprouting after striatal injury.
机译：对包括纹状体在内的中枢神经系统许多区域的伤害会导致伤口周围或“人工”发芽反应。为了直接评估巨噬细胞是否在刺激周围的发芽中起重要作用，对年轻时缺乏多种巨噬细胞亚型的骨石化（op / op）小鼠给予纹状体伤口，然后评估多巴胺能发芽的程度。损伤后两周，与对照组相比，op / op小鼠的纹状体中伤口巨噬细胞的数量明显减少（op / op小鼠为144 +/- 30.1，对照组为416.6 +/- 82.3，P <0.005，在横切伤口中间的部分）。多巴胺转运蛋白的免疫组织化学显示，op / op组动物的伤口周围发芽强度明显降低。使用[H3] -mazindol放射自显影对此效果进行量化，证实与对照组相比，op / op小鼠的创面发芽明显减少（op / op小鼠的蛋白质为71.4 +/- 21.7 fmol / mg，而210.7 +/- 27.1 fmol / mg对照蛋白质（P <0.0005）。在两组动物中，个体发芽反应的幅度与伤口巨噬细胞的数量密切相关（R = 0.83，R2 = 0.69）。我们的发现为巨噬细胞在纹状体损伤后诱导多巴胺能发芽中的关键参与提供了有力的支持。

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