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首页> 外文期刊>The European Journal of Neuroscience >Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells
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Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells

机译：CA1海马锥体细胞中三个钾通道的明显轴突-树突状分布

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摘要

Potassium channels comprise the most diverse family of ion channels and play critical roles in a large variety of physiological and pathological processes. In addition to their molecular diversity, variations in their distributions and densities on the axo-somato-dendritic surface of neurons are key parameters in determining their functional impact. Despite extensive electrophysiological and anatomical investigations, the exact location and densities of most K+ channels in small subcellular compartments are still unknown. Here we aimed at providing a quantitative surface map of two delayed-rectifier (Kv1.1 and Kv2.1) and one G-protein-gated inwardly rectifying (Kir3.2) K+ channel subunits on hippocampal CA1 pyramidal cells (PCs). Freeze-fracture replica immunogold labelling was employed to determine the relative densities of these K+ channel subunits in 18 axo-somato-dendritic compartments. Significant densities of the Kv1.1 subunit were detected on axon initial segments (AISs) and axon terminals, with an approximately eight-fold lower density in the latter compartment. The Kv2.1 subunit was found in somatic, proximal dendritic and AIS plasma membranes at approximately the same densities. This subunit has a non-uniform plasma membrane distribution; Kv2.1 clusters are frequently adjacent to, but never overlap with, GABAergic synapses. A quasi-linear increase in the Kir3.2 subunit density along the dendrites of PCs was detected, showing no significant difference between apical dendritic shafts, oblique dendrites or dendritic spines at the same distance from the soma. Our results demonstrate that each subunit has a unique cell-surface distribution pattern, and predict their differential involvement in synaptic integration and output generation at distinct subcellular compartments.
机译：钾离子通道包括最多样化的离子通道家族，并在多种生理和病理过程中发挥关键作用。除了它们的分子多样性外，神经元的轴突-躯体-树突表面上它们的分布和密度的变化是确定其功能影响的关键参数。尽管进行了广泛的电生理和解剖学研究，但在小亚细胞区室中大多数K +通道的确切位置和密度仍然未知。在这里，我们旨在提供海马CA1锥体细胞（PC）上两个延迟整流器（Kv1.1和Kv2.1）和一个G蛋白门控向内整流（Kir3.2）K +通道亚基的定量表面图。冷冻断裂复制品免疫金标记用于确定18个轴突-躯体-树突状区室中这些K +通道亚基的相对密度。在轴突起始节段（AIS）和轴突末端检测到Kv1.1亚基的显着密度，在后一个区室中密度降低了大约八倍。在体细胞，近端树突状细胞和AIS质膜中发现Kv2.1亚基的密度大致相同。该亚基的质膜分布不均匀; Kv2.1簇通常与GABA能突触相邻但绝不重叠。沿着PC的树突检测到Kir3.2亚基密度的近似线性增加，表明在距躯体相同距离处的顶端树突轴，斜树突或树突棘之间没有显着差异。我们的结果表明，每个亚基具有独特的细胞表面分布模式，并预测它们在突触整合和不同亚细胞区室的输出生成中的差异参与。

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