...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Neural changes accompanying tinnitus following unilateral acoustic trauma in the guinea pig
【24h】

Neural changes accompanying tinnitus following unilateral acoustic trauma in the guinea pig

机译：豚鼠单侧听觉外伤后伴随耳鸣的神经变化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Animal models of tinnitus allow us to study the relationship between changes in neural activity and the tinnitus percept. Here, guinea pigs were subjected to unilateral noise trauma and tested behaviourally for tinnitus 8 weeks later. By comparing animals with tinnitus with those without, all of which were noise-exposed, we were able to identify changes unique to the tinnitus group. Three physiological markers known to change following noise exposure were examined: spontaneous firing rates (SFRs) and burst firing in the inferior colliculus (IC), evoked auditory brainstem responses (ABRs), and the number of neurons in the cochlear nucleus containing nitric oxide synthase (NOS). We obtained behavioural evidence of tinnitus in 12 of 16 (75%) animals. Both SFRs and incidences of burst firing were elevated in the IC of all noise-exposed animals, but there were no differences between tinnitus and no-tinnitus animals. There were significant decreases in ipsilateral ABR latencies in tinnitus animals, contrary to what might be expected with a small hearing loss. Furthermore, there was an ipsilateral-contralateral asymmetry in NOS staining in the ventral cochlear nucleus (VCN) that was only apparent in tinnitus animals. Tinnitus animals had a significantly greater number of NOS-containing neurons on the noise-exposed side, whereas no-tinnitus animals did not. These data suggest that measuring NOS in the VCN and recording ABRs supplement behavioural methods for confirming tinnitus in animals, and that nitric oxide is involved in plastic neural changes associated with tinnitus. Tinnitus was induced in guinea pigs by exposing animals to loud noise, behaviourally identified, and compared with a series of neural markers. Spontaneous neuronal firing in the inferior colliculus increased after noise exposure but this did not correlate with behavioural tinnitus. Auditory brainstem response latencies were reduced in animals with tinnitus, while asymmetric nitric oxide synthase staining was identified in animals with tinnitus.
机译：耳鸣动物模型使我们能够研究神经活动变化与耳鸣知觉之间的关系。在这里，豚鼠遭受了单侧噪声损伤，并在8周后进行了耳鸣的行为测试。通过将带有耳鸣的动物与没有耳鸣的动物进行比较，所有这些动物都受到噪声的影响，我们能够识别出耳鸣组独特的变化。研究了三种已知的在噪声暴露后会发生变化的生理指标：自发放电频率（SFR）和下丘脑（IC）的突发放电，诱发的听觉脑干反应（ABR）以及含一氧化氮合酶的耳蜗核中神经元的数量（NOS）。我们获得了16只（75％）动物中12只耳鸣的行为证据。所有噪音动物的IC中，SFR和爆发射击的发生率均升高，但是耳鸣动物和无耳鸣动物之间没有差异。耳鸣动物的同侧ABR潜伏期显着减少，这与小听力损失的预期相反。此外，腹侧耳蜗核（VCN）的NOS染色存在同侧-对侧不对称性，仅在耳鸣动物中明显。耳鸣动物的噪音暴露侧明显多于含NOS的神经元，而无耳鸣动物则没有。这些数据表明，在VCN中测量NOS并记录ABR补充了确认动物耳鸣的行为方法，并且一氧化氮参与了与耳鸣有关的可塑性神经变化。豚鼠通过将动物暴露于大声噪音中，诱导其行为，并与一系列神经标志物进行比较，从而诱发了耳鸣。噪声暴露后，下丘的自发神经元放电增加，但这与行为性耳鸣无关。耳鸣动物的听觉脑干反应潜伏期缩短，而耳鸣动物则发现不对称的一氧化氮合酶染色。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号