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首页> 外文期刊>The European Journal of Neuroscience >Analysis of EphB receptors and their ligands in the developing retinocollicular system of the wallaby reveals dynamic patterns of expression in the retina.
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Analysis of EphB receptors and their ligands in the developing retinocollicular system of the wallaby reveals dynamic patterns of expression in the retina.

机译：分析小袋鼠发展中的视网膜胶体系统中的EphB受体及其配体，揭示了在视网膜中表达的动态模式。

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摘要

The expression of EphB1 and B2 receptors and ephrins-B1, -B2 and -B3 in the retina and superior colliculus of the wallaby (Macropus eugenii) was examined during the development of the retinocollicular projection, using reverse transcription-polymerase chain reaction and immunohistochemistry. There was an early transient differential expression of EphB2 that was higher in ventral retina and restricted to the outer neuroblast layer, whereas a high ventral to low dorsal gradient of ephrin-B2 expression occurred there throughout the study period. However, there was no dorsoventral gradient of receptors or ligands in retinal ganglion cells or a mediolateral gradient of ephrins in the colliculus. These findings suggest a limited role for these molecules in topographic mapping across the mediolateral colliculus in the wallaby. Early in retinal development there is a complementary pattern of expression of ephrin-B1 and -B2 in the outer neuroblast layer that overlaps with expression of EphB2. Ganglion and amacrine cells also express EphB2. As development proceeds subpopulations of putative horizontal and bipolar cells, also expressing EphB2, come to reside in the inner nuclear layer and ephrin-B1 is expressed throughout the outer nuclear layer. At the same time cells expressing ephrin-B2, and subpopulations of horizontal and bipolar cells come to reside in the inner nuclear layer and there is a corresponding decrease in ephrin-B2 expression in the outer nuclear layer. This pattern of coexpression of receptors and ligands suggests a role for them in cell migration and maintenance of laminar boundaries.
机译：使用逆转录-聚合酶链反应和免疫组化技术检测视网膜小关节投影发展过程中小袋鼠（europii eugenii）的视网膜和上丘中EphB1和B2受体以及ephrins-B1，-B2和-B3的表达。 EphB2的早期瞬时差异表达在腹侧视网膜中较高，并局限于外神经母细胞层，而在整个研究期间，那里的ephrin-B2表达出现了高腹侧至低背侧梯度。然而，在视网膜神经节细胞中没有受体或配体的背腹梯度，在胶体中没有ephrins的中外侧梯度。这些发现表明这些分子在鼠的中外侧胶体的地形图中的作用有限。在视网膜发育的早期，在神经母细胞外层中存在一种与EphB2重叠的互补表达形式，即ephrin-B1和-B2。神经节和无长突细胞也表达EphB2。随着发育的进行，假定的水平和双极细胞亚群（也表达EphB2）进入内核层，而ephrin-B1在整个外核层表达。同时，表达ephrin-B2的细胞以及水平和双极细胞的亚群都驻留在内部核层中，并且在外部核层中ephrin-B2的表达相应减少。受体和配体的这种共表达模式表明它们在细胞迁移和层流边界维持中的作用。

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