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首页> 外文期刊>The European Journal of Neuroscience >Active release of glycine or d-serine saturates the glycine site of NMDA receptors at the cerebellar mossy fibre to granule cell synapse.
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Active release of glycine or d-serine saturates the glycine site of NMDA receptors at the cerebellar mossy fibre to granule cell synapse.

机译：甘氨酸或d-丝氨酸的主动释放使小脑苔藓纤维上的NMDA受体的甘氨酸位点饱和到颗粒细胞突触。

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摘要

The current and calcium influx generated by NMDA receptors depend on the concentration of the coagonist glycine, or its analogue d-serine, in the synaptic cleft. If there is no release of glycine, the ionic stoichiometry of the glial GlyT1 glycine transporters expressed near NMDA receptors in the brain should be able to lower the extracellular glycine concentration to below the EC50 for coactivation of NMDA receptors. We examined whether changing the glycine or d-serine concentration in the superfusion solution altered the NMDA receptor mediated component of the synaptic current at the rat cerebellar mossy fibre to granule cell synapse. Adding up to 100 micro m glycine or d-serine had no effect, implying that the glycine site is saturated. Using the competitive glycine site antagonist 7-chlorokynurenate, and plausible values for the kinetic parameters of NMDA receptors, we estimate that during activation of the mossy fibres the concentration of glycine or d-serine in the synaptic cleft is at least 4.6 micro m or 1.5 micro m, respectively, requiring active release of glycine or d-serine.
机译：NMDA受体产生的电流和钙流入量取决于突触裂隙中激动剂甘氨酸或其类似物d-丝氨酸的浓度。如果没有释放甘氨酸，则在大脑中NMDA受体附近表达的神经胶质GlyT1甘氨酸转运蛋白的离子化学计量应能够将胞外甘氨酸浓度降低至EC50以下，以使NMDA受体共激活。我们检查了改变融合溶液中甘氨酸或d-丝氨酸的浓度是否改变了大鼠小脑苔藓纤维到颗粒细胞突触的NMDA受体介导的突触电流成分。最多添加100微米的甘氨酸或d-丝氨酸无效，这表明甘氨酸位点已饱和。使用竞争性甘氨酸位点拮抗剂7-氯基尿酸，以及NMDA受体动力学参数的合理值，我们估计在苔藓纤维活化过程中，突触间隙中甘氨酸或d-丝氨酸的浓度至少为4.6微米或1.5微米，分别需要主动释放甘氨酸或d-丝氨酸。

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