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首页> 外文期刊>The European Journal of Neuroscience >Analysis of differential gene expression supports a role for amyloid precursor protein and a protein kinase C substrate (MARCKS) in long-term memory.
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Analysis of differential gene expression supports a role for amyloid precursor protein and a protein kinase C substrate (MARCKS) in long-term memory.

机译：差异基因表达的分析支持淀粉样蛋白前体蛋白和蛋白激酶C底物（MARCKS）在长期记忆中的作用。

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摘要

Previous work has identified the intermediate and medial part of the hyperstriatum ventrale (IMHV) as a region of the chick brain storing information acquired through the learning process of imprinting. We have examined in this brain region changes in expression of candidate genes involved in memory. Chicks were exposed to a rotating red box and the strength of their preference for it, a measure of learning, determined. Brain samples were removed approximately 24 h after training. Candidate genes whose expressions were different in IMHV samples derived from strongly imprinted chicks relative to those from chicks showing little or no learning were identified using subtractive hybridization. The translation products of two candidate genes were investigated further in samples from the left and right IMHV and from two other brain regions not previously implicated in imprinting, the left and right posterior neostriatum. One of the proteins was the amyloid precursor protein (APP), the other was myristoylatedalanine rich C kinase substrate (MARCKS). In the left IMHV the levels of the two proteins increased with the strength of learning. The effects in the right IMHV were not significantly different from those in the left. There were no effects of learning in the posterior neostriatum. This is the first study to relate changes in the amounts of MARCKS and APP proteins to the strength of learning in a brain region known to be a memory store and demonstrates that the systematic identification of protein molecules involved in memory formation is possible.
机译：先前的工作已将腹侧上皮纹状体（IMHV）的中间和内侧部分确定为存储通过印记学习过程获得的信息的小鸡大脑区域。我们已经检查了该大脑区域中参与记忆的候选基因表达的变化。将小鸡暴露在旋转的红色盒子中，并确定它们对它的喜好程度（学习程度）。训练后约24小时取出脑样本。使用消减杂交，鉴定了在强印迹动物的IMHV样品中表达相对于那些学习很少或没有学习的鸡表达不同的候选基因。在左右IMHV以及先前未涉及印迹的其他两个大脑区域（左后和右后新纹状体）的样品中进一步研究了两个候选基因的翻译产物。一种蛋白质是淀粉样蛋白前体蛋白质（APP），另一种是富含肉豆蔻酰化丙氨酸的C激酶底物（MARCKS）。在左侧的IMHV中，两种蛋白质的水平随学习强度的增加而增加。右侧IMHV的效果与左侧的效果无显着差异。新纹状体后部没有学习的影响。这是第一个将MARCKS和APP蛋白质的量变化与大脑区域（称为记忆存储区）的学习强度相关的研究，并证明了系统识别参与记忆形成的蛋白质分子是可能的。

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