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首页> 外文期刊>The European Journal of Neuroscience >Subtype switching of vesicular glutamate transporters at parallel fibre-Purkinje cell synapses in developing mouse cerebellum.
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Subtype switching of vesicular glutamate transporters at parallel fibre-Purkinje cell synapses in developing mouse cerebellum.

机译：发育中的小鼠小脑中平行纤维-Purkinje细胞突触处水泡谷氨酸转运蛋白的亚型转换。

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摘要

Two subtypes of the vesicular glutamate transporter are expressed differentially in two excitatory afferents synapsing on to Purkinje cells: VGluT1 (BNPI) in axon terminals of cerebellar granule cells (i.e. parallel fibres; PFs) and VGluT2 (DNPI) in those of the inferior olivary neurons (climbing fibres; CFs). In the present study, we examined their expression in the developing mouse cerebellum. By in situ hybridization, the inferior olivary nucleus selectively expressed VGluT2 mRNA through postnatal life. In the cerebellum, both subtypes were transcribed in the external and internal granular layers during the first postnatal week. Thereafter, VGluT1 mRNA showed marked upregulation in the internal granular layer, whereas VGluT2 mRNA disappeared from the external and internal granular layers by the end of the third postnatal week. By immunohistochemistry, CF terminals consistently exhibited VGluT2 immunoreactivity in the postnatal cerebellum. By contrast, in the first 10 days of postnatal life, VGluT2 predominated in PF terminals, despite the transcription of both transporters in developing granule cells. During the second 10 days, VGluT2 in PF terminals was replaced with VGluT1 from deep regions of the molecular layer upwards, correlating with dendritic translocation of CFs. This replacement was accomplished by postnatal day 30. Taking that late-borne PFs are laid down successively on earlier ones in the molecular layer, the deep-to-superficial replacement represents maturation-linked switching from VGluT2 to VGluT1 in individual PFs, and is likely to be regulated at both the transcription and translation levels.
机译：两种水泡谷氨酸转运蛋白亚型在突触到Purkinje细胞的两个兴奋性传入神经元中差异表达：小脑颗粒细胞（即平行纤维; PFs）的轴突末端的VGluT1（BNPI）和下橄榄核神经元的VGluT2（DNPI）。 （攀爬纤维; CF）。在本研究中，我们检查了它们在发育中的小鼠小脑中的表达。通过原位杂交，下橄榄核在产后生活中选择性表达VGluT2 mRNA。在小脑中，两个亚型在产后第一周都在外部和内部颗粒层转录。此后，VGluT1 mRNA在内部颗粒层中显示出明显的上调，​​而VGluT2 mRNA在出生后第三周结束时从外部和内部颗粒层中消失。通过免疫组织化学，CF末端在产后小脑中始终表现出VGluT2免疫反应性。相比之下，在出生后的前10天，尽管两种转运蛋白在发育中的颗粒细胞中都有转录，但VGluT2在PF末端占主导地位。在接下来的10天中，从分子层深处向上的PF末端的VGluT2被VGluT1取代，这与CF的树突状移位有关。这种替换是在出生后30天完成的。考虑到将晚期传播的PFs依次放置在分子层中较早的PFs上，从深到浅的替换代表了各个PFs中从VGluT2到VGluT1的成熟连锁转换。在转录和翻译水平上均受调控。

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