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首页> 外文期刊>The European Journal of Neuroscience >Reversible modulation of GABAA receptor-mediated currents by light is dependent on the redox state of the receptor.
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Reversible modulation of GABAA receptor-mediated currents by light is dependent on the redox state of the receptor.

机译：光对GABA A受体介导的电流的可逆调节取决于受体的氧化还原状态。

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摘要

Light has recently been shown to be a physical modulator of GABAA receptor activity. Here, we further characterize the effects of light on a native cortical and retinal population of GABAA receptors, and identify a possible mechanism for light induced potentiation using recombinant receptors. GABA-induced currents in cortical neurons were observed to be rapidly and reversibly potentiated following exposure to a brief flash of light (0.5-2 s; > 280 nm) directed via an optical fibre (50 micro m i.d.). GABAA receptor-mediated responses in retinal ganglion cells were also enhanced by light, while glycine-induced currents in these cells were unaffected by the same stimulus. We also determined that physiological levels of light, that is, those that would normally reach the retina, also enhanced GABA-induced currents. Finally, we observed that chemical reduction of recombinant alpha1beta2 and alpha1beta2gamma2S GABAA receptors by dithiothreitol substantially attenuated the effects of light. These results suggest that GABAA receptors can be reversibly modified by a brief pulse of light via an allosteric mechanism that is intimately linked to redox modulation.
机译：最近显示光是GABAA受体活性的物理调节剂。在这里，我们进一步表征光对GABA A受体的天然皮质和视网膜群体的影响，并确定使用重组受体的光诱导增强作用的可能机制。观察到GABA诱导的皮层神经元电流在暴露于通过光纤（50 micro m.d.）发出的短暂闪光（0.5-2 s;> 280 nm）之后迅速且可逆地增强。视网膜神经节细胞中的GABA A受体介导的反应也被光增强，而甘氨酸诱导的这些细胞中的电流不受相同刺激的影响。我们还确定，生理水平的光，即正常情况下会到达视网膜的光，也可以增强GABA诱导的电流。最后，我们观察到二硫苏糖醇对重组alpha1beta2和alpha1beta2gamma2S GABAA受体的化学还原作用大大减弱了光的影响。这些结果表明，通过与氧化还原调节密切相关的变构机制，短暂的光脉冲可以可逆地修饰GABA A受体。

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