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首页> 外文期刊>The European Journal of Neuroscience >Subcellular distribution of L-type Ca2+ channels responsible for plateau potentials in motoneurons from the lumbar spinal cord of the turtle.
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Subcellular distribution of L-type Ca2+ channels responsible for plateau potentials in motoneurons from the lumbar spinal cord of the turtle.

机译：L型Ca2 +通道的亚细胞分布负责海龟腰脊髓运动神经元的平台电位。

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摘要

L-type calcium channels mediate the persistent inward current underlying plateau potentials in spinal motoneurons. Electrophysiological analysis shows that plateau potentials are generated by a persistent inward current mediated by low threshold L-type calcium channels located in the dendrites. As motoneurons express L-type calcium channels of the CaV1.2 and CaV1.3 subtypes, we have investigated the subcellular distribution of these channels using antibody labelling. The plateau generating a persistent inward current is modulated by the activation of metabotropic receptors. For this reason, we also examined the relationship between CaV1.2 and CaV1.3 subunits in motoneurons and presynaptic terminals labelled with antibodies against synapsin 1a. Motoneurons in the spinal cord of the adult turtle were identified as large neurons, immunopositive for choline acetyltransferase, located in the ventral horn. In these neurons, CaV1.2 subunits were present in the cell bodies and axons. Patches of CaV1.3 subunitswere seen in association with the cell membrane of the somata and both the proximal and distal dendrites. Double labelling with an antibody against synapsin 1a showed that CaV1.3 subunits, but not CaV1.2 subunits, were always located at synaptic sites. The distribution of CaV1.2 and CaV1.3 strongly suggests that the persistent inward current underlying plateau potentials in spinal motoneurons is mediated by CaV1.3 and not by CaV1.2. Our findings also show that CaV1.3 may be located in the somatic and dendritic membrane adjacent to particular presynaptic terminals.
机译：L型钙通道介导脊髓运动神经元中平台电位的持续内向电流。电生理分析表明，平台电位是由位于树突中的低阈值L型钙通道介导的持续向内电流产生的。由于运动神经元表达CaV1.2和CaV1.3亚型的L型钙通道，我们使用抗体标记研究了这些通道的亚细胞分布。产生持续的内向电流的平台通过代谢型受体的激活来调节。因此，我们还检查了运动神经元中CaV1.2和CaV1.3亚基与被突触蛋白1a抗体标记的突触前末端之间的关系。成年乌龟脊髓中的动子素被鉴定为位于腹角的大神经元，胆碱乙酰转移酶免疫阳性。在这些神经元中，CaV1.2亚基存在于细胞体和轴突中。可见到CaV1.3亚基的斑块与躯体的细胞膜以及近端和远端树突相关。用抗突触蛋白1a的抗体进行双重标记显示，CaV1.3亚基而不是CaV1.2亚基始终位于突触位点。 CaV1.2和CaV1.3的分布强烈表明，脊髓运动神经元中持续的内向高原电位是由CaV1.3介导的，而不是由CaV1.2介导的。我们的发现还表明，CaV1.3可能位于特定突触前末端附近的体细胞和树突状膜中。

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