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首页> 外文期刊>The European Journal of Neuroscience >Bicuculline induces synapse formation on primary cultured accessory olfactory bulb neurons.
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Bicuculline induces synapse formation on primary cultured accessory olfactory bulb neurons.

机译：Bicuculline在原代培养的嗅球神经元上诱导突触形成。

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摘要

To investigate the roles of the GABAergic inhibitory system of accessory olfactory bulb (AOB) in pheromonal memory formation, we have developed a primary culture system of AOB neurons, which had numerous excitatory and inhibitory synapses. Using this culture system of AOB neurons, we examined the correlation in rats between neuronal excitation and synaptic morphology by bicuculline-induced disinhibition of cultured AOB neurons. The exposure to bicuculline induced long-lasting oscillatory changes in the intracellular calcium level ([Ca2+]in) of cultured non-GABAergic multipolar neurons, which were identified as mitral/tufted cells (MT cells). These MT cells exhibited the appearance of dendritic filopodia structures after a 10-min treatment with bicuculline. By labelling presynaptic terminals with FM4-64, the appearance of new presynaptic terminals was clearly observed on newly formed filopodia after 120 min treatment with bicuculline. These results suggest that bicuculline-induced [Ca2+]in oscillation of MT cells induces the growth of filopodia and subsequently the formation of new presynaptic terminals. Furthermore, tetrodotoxin or the deprivation of extracellular calcium blocked bicuculline-induced synapse formation. The present results indicate that the long-lasting [Ca2+]in oscillation caused by bicuculline-induced disinhibition of cultured MT cells is significantly implicated in the mechanism underlying synapse formation on cultured AOB neurons. Our established culture system of AOB neurons will aid in clarifying the mechanism of synapse formation between AOB neurons and the molecular mechanism of pheromonal memory formation.
机译：为了研究辅助嗅球（AOB）的GABA能抑制系统在信息素记忆形成中的作用，我们开发了具有许多兴奋性和抑制性突触的AOB神经元的原代培养系统。使用这种AOB神经元的培养系统，我们通过双卵磷脂诱导的培养的AOB神经元的去抑制作用，检查了大鼠神经元兴奋与突触形态之间的相关性。暴露于比库卡林可引起培养的非GABA能的多极神经元的细胞内钙水平（[Ca2 +] in）的长期振荡变化，这些变化被确定为二尖瓣/簇状细胞（MT细胞）。在用双瓜氨酸处理10分钟后，这些MT细胞表现出树突状丝状伪足结构的外观。通过用FM4-64标记突触前末端，在用小花碱处理120分钟后，新形成的丝状伪足清晰地观察到新的突触前末端的出现。这些结果表明，双小分子诱导的MT细胞的[Ca2 +]振荡诱导丝状伪足的生长，并随后形成新的突触前末端。此外，河豚毒素或细胞外钙的缺乏阻止了双小分子诱导的突触形成。目前的结果表明，由双小分子诱导的培养MT细胞抑制作用引起的[Ca2 +]的长期振荡与培养的AOB神经元突触形成的机制密切相关。我们建立的AOB神经元培养系统将有助于阐明AOB神经元之间突触形成的机制和信息素记忆形成的分子机制。

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