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首页> 外文期刊>The European Journal of Neuroscience >Associative mossy fibre LTP induced by pairing presynaptic stimulation with postsynaptic hyperpolarization of CA3 neurons in rat hippocampal slice.
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Associative mossy fibre LTP induced by pairing presynaptic stimulation with postsynaptic hyperpolarization of CA3 neurons in rat hippocampal slice.

机译：突触前刺激与大鼠海马切片中CA3神经元的突触后超极化配对诱导的联想苔藓纤维LTP。

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摘要

Whole cell recordings of excitatory postsynaptic potentials/currents (EPSPs/EPSCs) evoked by minimal stimulation of commissural-associative (CF) and mossy fibre (MF) inputs were performed in CA3 pyramidal neurons. Paired responses (at 50 ms intervals) were recorded before, during and after hyperpolarization of the postsynaptic membrane (20-30 mV for 15-35 min). Membrane hyperpolarization produced a supralinear increase of EPSPs/EPSCs amplitude in MF-inputs. Synaptic responses remained potentiated for the rest of the recording period (up to 40 min) after resetting the membrane potential to control level (221 +/- 60%, n = 15 and 219 +/- 61%, n = 11 for MF-EPSP and MF-EPSC, respectively). We shall refer to this effect as hyperpolarization-induced LTP (HI-LTP). In the absence of afferent stimulation, membrane hyperpolarization was unable to produce HI-LTP. In contrast to MF-EPSPs, the mean amplitude of CF-EPSPs did not increase significantly after hyperpolarization relative to controls (138 +/- 29%, n = 22). HI-LTP was associated with modifications of classical indices of presynaptic release: paired-pulse facilitation, failures rate, coefficient of variation of EPSP amplitudes and quantal content. The induction of HI-LTP was NMDA independent but was dependent on metabotropic glutamate receptors (mGluRs) activation and calcium release from inositol 1,4,5-triphosphate (IP3)-sensitive intracellular stores: it was prevented by mGluR antagonist, intracellular heparin and BAPTA. We conclude that while the induction of HI-LTP was postsynaptic, its expression was presynaptic.
机译：在CA3锥体神经元中，通过对联结缔合（CF）和苔藓纤维（MF）输入的最小刺激而诱发的兴奋性突触后电位/电流（EPSP / EPSC）的全细胞记录。在突触后膜超极化之前，期间和之后（20-30 mV持续15-35分钟），记录配对响应（以50 ms间隔）。膜超极化使MF输入中的EPSP / EPSC幅度超线性增加。在将膜电势重置为控制水平（MF-221为221 +/- 60％，n = 15和219 +/- 61％，n = 11）后的整个记录​​期间（最长40分钟），突触反应仍保持增强状态。 EPSP和MF-EPSC）。我们将这种效应称为超极化诱导的LTP（HI-LTP）。在没有传入刺激的情况下，膜超极化不能产生HI-LTP。与MF-EPSP相比，超极化后CF-EPSP的平均幅度相对于对照组没有明显增加（138 +/- 29％，n = 22）。 HI-LTP与突触前释放的经典指标的改变有关：成对脉冲促进，失败率，EPSP振幅变化系数和定量含量。 HI-LTP的诱导与NMDA无关，但取决于代谢型谷氨酸受体（mGluRs）的活化和钙从肌醇1,4,5-三磷酸（IP3）敏感的细胞内存储中释放：它被mGluR拮抗剂，细胞内肝素和BAPTA。我们得出的结论是，虽然HI-LTP的诱导是突触后的，但其表达是突触前的。

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