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首页> 外文期刊>The European Journal of Neuroscience >Striatal neurons in striatal grafts are derived from both post-mitotic cells and dividing progenitors.
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Striatal neurons in striatal grafts are derived from both post-mitotic cells and dividing progenitors.

机译：纹状体移植物中的纹状体神经元既来自有丝分裂后细胞，又来自分裂祖细胞。

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摘要

Transplants of embryonic striatal tissue are characteristically heterogeneous, containing patches (P-zones) of striatal medium spiny projection neurons. It is not yet known how this morphology develops, and whether the striatal neurons in the grafts are derived from post-mitotic neuroblasts in the embryonic brain or from striatal progenitors that continue to divide after transplantation. To address this question we labelled dividing cells in the transplants with bromodeoxyuridine (BrdU), either prior to or after transplantation into the adult lesioned rat striatum. Cells for transplantation were either pre-labelled in utero by intraperitoneal (i.p.) injections of BrdU, or post-labelled after transplantation by i.p. injections to the hosts. Either two or six months after transplantation the brains were processed using double immunohistochemical techniques to detect BrdU and calbindin-positive neurons in the transplants. In the transplants pre-labelled with BrdU, approximately 30% of calbindin-positive cells were heavily labelled with BrdU, suggesting these had undergone a final division prior to transplantation. In transplants where cells had been labelled post-transplantation, approximately 17% of calbindin cells were heavily BrdU labelled. These results suggest that whereas a proportion of striatal medium spiny neurons in the striatal grafts were post-mitotic at the time of transplantation, other striatal progenitor cells can continue to divide after transplantation, and then complete an appropriate neuronal maturation programme in the adult host brain environment.
机译：胚胎纹状体组织的移植物特征上是异质的，包含纹状体中棘突状投射神经元的斑块（P区）。尚不知道这种形态如何发展，以及移植物中的纹状体神经元是源自胚胎脑中的有丝分裂后神经母细胞，还是源自在移植后继续分裂的纹状体祖细胞。为了解决这个问题，我们在移植到成年病变大鼠纹状体之前或之后，用溴脱氧尿苷（BrdU）标记了移植物中的分裂细胞。通过腹膜内（i.p.）注射BrdU在子宫内预先标记要移植的细胞，或在i.p.移植后再标记子宫内。注射到主机。移植后两到六个月，使用双重免疫组织化学技术对大脑进行处理，以检测移植物中的BrdU和calbindin阳性神经元。在预先标记有BrdU的移植物中，大约30％的钙结合蛋白阳性细胞被BrdU大量标记，这表明这些细胞在移植前已经历了最终分裂。在移植后细胞已被标记的移植物中，约有17％的钙结合蛋白细胞被BrdU高度标记。这些结果表明，尽管纹状体移植物中一部分纹状体中棘神经元在移植时有丝分裂，但其他纹状体祖细胞在移植后仍可以继续分裂，然后在成年宿主脑中完成适当的神经元成熟程序环境。

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