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首页> 外文期刊>The European Journal of Neuroscience >Glutamatergic modulation of synaptic plasticity at a PNS vertebrate cholinergic synapse.
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Glutamatergic modulation of synaptic plasticity at a PNS vertebrate cholinergic synapse.

机译：PNS脊椎动物胆碱能突触的突触可塑性的谷氨酸调节。

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The presence and the functionality of a glutamatergic regulation was studied at the frog neuromuscular junction (NMJ), a singly innervated cholinergic synapse. Bath application of glutamate reduced transmitter release without affecting nerve-evoked presynaptic Ca2+ entry and handling. (1S,3R)-aminocyclopentanedicarboxylic acid (ACPD), a metabotropic glutamate receptor (mGluR) agonist, mimicked the effects of glutamate while (S)-alpha-methyl-4-carboxyphenylglycine (MCPG), a mGluR antagonist, blocked glutamate effects. MCPG had no effect on transmitter release evoked at low frequency (0.2 Hz) but significantly reduced synaptic depression (10 Hz, 80 s). This suggests that a frequency-dependent endogenous glutamatergic modulation is present at the frog NMJ and is mediated through mGluRs. Immunohistochemical labelling revealed the presence of mGluRs at the end plate area, primarily on muscle fibers. Functional glutamate uptake machinery was also found at the NMJ as blockade of glutamate transport by the inhibitor dl-threo-beta-benzyloxyaspartate (DL-TBOA) increased high frequency-induced depression, suggesting that the transporters system is used to eliminate glutamate from the extracellular space. Moreover, immunohistochemical labelling revealed that glutamate-aspartate transporters (GLASTs) are predominantly present on perisynaptic Schwann cells (PSCs). However, local application of glutamate on PSCs unreliability evoked small Ca2+ responses. Hence, these data suggest that functional glutamatergic interactions at a purely cholinergic synapse, shape synaptic efficacy and short-term plasticity in a frequency-dependent fashion.
机译：在单个神经支配的胆碱能突触青蛙神经肌肉接头（NMJ）中研究了谷氨酸能调节的存在和功能。谷氨酸的沐浴应用减少了递质的释放，而不会影响神经诱发的突触前钙离子的进入和处理。代谢型谷氨酸受体（mGluR）激动剂（1S，3R）-氨基环戊二羧酸（ACPD）模仿了谷氨酸的作用，而mGluR拮抗剂（S）-α-甲基-4-羧苯基甘氨酸（MCPG）阻止了谷氨酸的作用。 MCPG对低频（0.2 Hz）引起的递质释放没有影响，但显着降低了突触抑制（10 Hz，80 s）。这表明在青蛙NMJ处存在频率依赖性内源性谷氨酸能调节，并通过mGluRs介导。免疫组织化学标记显示在终板区域主要在肌肉纤维上存在mGluRs。在NMJ处还发现了功能性谷氨酸摄取机制，这是由于抑制剂dl-苏式-β-苄氧基天冬氨酸（DL-TBOA）增加了高频诱导的抑郁，从而阻止了谷氨酸的转运，这表明转运蛋白系统可用于从细胞外清除谷氨酸。空间。此外，免疫组化标记显示，谷氨酸-天冬氨酸转运蛋白（GLASTs）主要存在于突触周围的雪旺细胞（PSC）上。但是，在不可靠的PSC上局部应用谷氨酸盐会引起小的Ca2 +反应。因此，这些数据表明，纯胆碱能突触的功能性谷氨酸能相互作用，形状突触功效和短期可塑性以频率依赖性方式存在。

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