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首页> 外文期刊>The European Journal of Neuroscience >Light-induced c-fos in melanopsin retinal ganglion cells of young and aged rodless/coneless (rd/rd cl) mice.
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Light-induced c-fos in melanopsin retinal ganglion cells of young and aged rodless/coneless (rd/rd cl) mice.

机译：年轻和年老的无杆/无锥（rd / rd cl）小鼠的黑视蛋白视网膜神经节细胞中的光诱导c-fos。

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Non-rod, non-cone ocular photoreceptors have been shown to mediate a range of irradiance detection tasks. The strongest candidates for these receptors are melanopsin-positive retinal ganglion cells (RGCs). To provide a more complete understanding of these receptors in vivo, we have utilized a mouse that lacks rod and cone photoreceptors (rd/rd cl) and compared these animals to congenic wild-types. Using real-time polymerase chain reaction and immunohistochemistry, we address the following. (1) Is Fos expression within these RGCs driven by an input from the rods/cones or is it the product of the intrinsic photosensitivity of these neurons? We demonstrate that most Fos expression across the entire retina is due to the rods/cones, but in the absence of these photoreceptors, light will induce Fos within melanopsin RGCs. (2) Could the reported age-related decline in circadian photosensitivity of rodents be linked to changes in the population of melanopsin RGCs? We show that old mice experience an approximately 40% reduction in melanopsin RGCs. (3) Does the loss of inner retinal neurons affect the responses of melanopsin RGCs? Aged ( approximately 700 days) rd/rd cl mice lose most of their inner retina but retain the retinal ganglion cell layer. In these mice, the proportion of melanopsin RGCs that express Fos in response to light is significantly reduced. Collectively, our data suggest that melanopsin RGCs form a heterogeneous population of neurons, and that most of the light-induced c-fos expression within these cells is associated with the endogenous photosensitivity of these neurons.
机译：非杆状，非圆锥形的眼感光体已被证明可介导一系列辐照度检测任务。这些受体最强的候选者是黑视蛋白阳性的视网膜神经节细胞（RGC）。为了提供对这些受体在体内的更完整的了解，我们利用了缺少杆和锥光感受器（rd / rd cl）的小鼠，并将这些动物与同基因野生型进行了比较。使用实时聚合酶链反应和免疫组化，我们解决以下问题。 （1）这些RGC中的Fos表达是受杆/锥输入的驱动还是它是这些神经元固有光敏性的产物？我们证明了整个视网膜上大多数Fos表达是由于视杆/视锥细胞引起的，但是在没有这些感光器的情况下，光会在黑色素RGCs中诱导Fos。 （2）啮齿类动物的昼夜节律敏感性与年龄相关的报道是否与黑色素RGC种群的变化有关？我们显示，老年小鼠的黑素视蛋白RGC减少约40％。 （3）视网膜内神经元的丧失会影响黑视蛋白RGC的反应吗？年龄（约700天）的rd / rd cl小鼠失去了大部分的内部视网膜，但保留了视网膜神经节细胞层。在这些小鼠中，响应光表达Fos的黑色素RGC的比例显着降低。总体而言，我们的数据表明，黑视蛋白RGC形成神经元的异质群体，并且这些细胞内大多数光诱导的c-fos表达与这些神经元的内源光敏性有关。

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