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首页> 外文期刊>The European Journal of Neuroscience >Migration and differentiation of neural progenitor cells from two different regions of embryonic central nervous system after transplantation into the intact spinal cord.
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Migration and differentiation of neural progenitor cells from two different regions of embryonic central nervous system after transplantation into the intact spinal cord.

机译：胚胎中枢神经系统移植后，来自胚胎中枢神经系统两个不同区域的神经祖细胞的迁移和分化。

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摘要

Transplantation of in vitro-expanded neural stem cells (NSCs) is a potentially powerful tool to repair functions of the injured spinal cord. A prerequisite for the successful transplantation therapy is identification of optimized experimental parameters that can promote maximal survival, extensive migration and selective differentiation of the transplanted NSC population in the spinal cord. To this end, we evaluated the basic characteristics of NSC-like cells from two different donor sources, the embryonic hippocampus and spinal cord, after transplantation into the neonatal spinal cord. Proliferation and differentiation phenotypes of both NSC-like cells can be controlled by the concentration of fibroblast growth factor-2 (FGF-2) in vitro. Both NSC-like cells can survive within the environment of the intact neonatal spinal cord and showed extensive migratory behaviour shortly after transplantation. However, quantitative analysis revealed preferential migration of hippocampus-derived cells in the dorsal white matter. Both NSC-like cells showed restricted phenotype toward the oligodendroglial lineage after transplantation. Transplantation of the mixture of two cell types revealed selective survival of hippocampus-derived NSC-like cells. This study indicates the possibility of transplanting hippocampus-derived NSCs to supply the cell source for immature oligodendrocytes, which are thought to be essential for both the myelination and trophic support of regenerating axons in the dorsal white matter of the spinal cord.
机译：体外扩增的神经干细胞（NSC）的移植是修复受损脊髓功能的潜在强大工具。成功进行移植治疗的前提是确定优化的实验参数，这些参数可以促进脊髓中NSC种群的最大存活率，广泛迁移和选择性分化。为此，我们评估了两种不同供体来源的NSC样细胞的基本特征，这些细胞来自胚胎海马和脊髓，并已移植到新生儿脊髓中。两种NSC样细胞的增殖和分化表型都可以通过体外成纤维细胞生长因子2（FGF-2）的浓度来控制。两种NSC样细胞都可以在完整的新生儿脊髓环境中生存，并且在移植后不久即表现出广泛的迁徙行为。但是，定量分析显示，海马源细胞在背白质中优先迁移。两种NSC样细胞在移植后均表现出对少突神经胶质谱系的限制性表型。两种细胞类型的混合物的移植揭示了海马来源的NSC样细胞的选择性存活。这项研究表明，移植海马神经干细胞可以为未成熟的少突胶质细胞提供细胞来源，据认为这对于脊髓背白质中轴突再生的髓鞘化和营养支持都是必不可少的。

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