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首页> 外文期刊>The European Journal of Neuroscience >Brain capillaries and cholinergic neurons persist in organotypic brain slices in the absence of blood flow.
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Brain capillaries and cholinergic neurons persist in organotypic brain slices in the absence of blood flow.

机译：在没有血流的情况下，脑毛细血管和胆碱能神经元持续存在于器官型脑切片中。

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摘要

Angiogenesis plays an important role during development of the brain and under pathological conditions. The aim of the present study was to observe interaction of brain capillaries and cholinergic neurons in organotypic brain slices. Immunohistochemistry was used to visualize brain capillary-like structures (RECA-1 antigen) and cholinergic neurons (choline acetyltransferase). Under normal culture conditions, a very low number of brain capillaries was found in 2- and 4-week-old cortex brain slices. Treatment of slices with acidic medium (pH 6) or hyperthermia (42 degrees C) markedly enhanced the number of brain capillaries. Incubation with 10 ng/mL vascular endothelial growth factor only enhanced angiogenesis in more developed slices. Cholinergic neurons survived in slices of the basal nucleus of Meynert; however, hyperthermia but not acidosis markedly decreased their number. In coslices of the basal nucleus of Meynert and cortex (pretreated with acidic medium), a high number of RECA-1-positive capillaries and cholinergic neurons persisted and displayed strong nerve fibre growth of cholinergic fibres into the cortex. In conclusion, we have demonstrated that RECA-1-positive capillaries and cholinergic neurons can be studied in slice cultures in the absence of blood perfusion, and that this model could provide a system to study mechanisms involved in vascular dementia.
机译：血管生成在大脑发育过程中和病理条件下起着重要作用。本研究的目的是观察器官型脑切片中脑毛细血管和胆碱能神经元的相互作用。免疫组织化学用于可视化脑毛细血管样结构（RECA-1抗原）和胆碱能神经元（胆碱乙酰基转移酶）。在正常培养条件下，在2周龄和4周龄的皮质大脑切片中发现的脑毛细血管数量非常少。用酸性介质（pH 6）或高温（42摄氏度）处理切片可显着增强脑毛细血管的数量。与10 ng / mL血管内皮生长因子一起孵育只会增强较发达切片中的血管生成。胆碱能神经元在Meynert的基底核切片中存活;然而，热疗而不是酸中毒明显减少了其数量。在Meynert和皮层（用酸性介质预处理）的基核的皮层中，大量RECA-1阳性毛细血管和胆碱能神经元持续存在，并显示胆碱能纤维向皮层的强烈神经纤维生长。总之，我们证明了在没有血液灌注的情况下，可以在切片培养物中研究RECA-1阳性的毛细血管和胆碱能神经元，并且该模型可以为研究涉及血管性痴呆的机制提供系统。

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