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首页> 外文期刊>The European Journal of Neuroscience >Spinal cord plasticity in response to unilateral inhibition of the rat motor cortex during development: changes to gene expression, muscle afferents and the ipsilateral corticospinal projection.
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Spinal cord plasticity in response to unilateral inhibition of the rat motor cortex during development: changes to gene expression, muscle afferents and the ipsilateral corticospinal projection.

机译：响应发育过程中大鼠运动皮质的单方面抑制而产生的脊髓可塑性：基因表达，肌肉传入和同侧皮质脊髓投射的变化。

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Abstract In developing Wistar albino rats, ventral horn muscle afferent boutons are lost following corticospinal innervation. Motor cortex lesions rescue a proportion of these boutons and perturb activity dependent expression of cJun and parvalbumin (PV) in the spinal cord. Therefore, we tested whether activity-dependent competition between corticospinal and proprioreceptive afferents determines the balance of these inputs to motor output pathways by delivering the inhibitory GABA agonist muscimol unilaterally to the forelimb motor cortex using slow release polymer implants from postnatal day 7 (P7) coincident with corticospinal synaptogenesis. Controls received saline. Inhibition of immature cortical neurons by muscimol was confirmed with separate in vitro electrophysiological recordings. After P28, spinal cord sections were immunostained for PV, cJun and muscle afferents transganglionically labelled with cholera toxin-B (CTB). Unilateral inhibition reduced contralaterally the number of PV positive spinal cord neurons and muscle afferent boutons in the dorsolateral ventral horn, compared to controls, and significantly altered the distribution of motoneuronal cJun expression. Separately, descending tracts were retrogradely traced with CTB from the cervical hemicord contralateral to implants. Forelimb sensorimotor cortex sections were immunostained for either CTB or PV. In muscimol treated animals, significantly fewer neurons expressed PV in the inhibited hemicortex, but as many CTB labelled corticospinal neurons were present as in controls, along with an equally large corticospinal projection from contralateral to the implant, significantly greater than in controls. Unexpectedly, unilateral inhibition of the motor cortical input did not lead to an expanded muscle afferent input. Instead, this was reduced coincident with development of a bilateral corticospinal innervation.
机译：摘要在发育中的Wistar白化病大鼠中，皮质脊髓神经支配后，腹角肌肉传入的bouton丢失。运动皮层损伤可挽救一部分这种钮扣，并在脊髓中干扰cJun和小白蛋白（PV）的活动，并依赖于其表达。因此，我们测试了出生后第7天（P7）巧合地通过使用缓释聚合物植入物将抑制性GABA激动剂麝香酚单侧递送至前肢运动皮层，从而确定皮质脊髓和本体感受传入者之间的活动依赖性竞争是否决定了这些输入与运动输出路径的平衡与皮质脊髓突触发生有关。对照接受盐水。单独的体外电生理学记录证实了麝香酚对未成熟皮质神经元的抑制作用。 P28后，对脊髓切片进行免疫染色，对霍乱毒素B（CTB）进行神经节标记的PV，cJun和肌肉传入神经。与对照组相比，单侧抑制使对侧减少了背外侧腹角中PV阳性脊髓神经元和肌肉传入的bouton的数量，并显着改变了运动神经元cJun表达的分布。另外，CTB从种植体的对侧颈对侧逆行追踪下降道。对前肢感觉运动皮层切片进行CTB或PV免疫染色。在使用麝香酚治疗的动物中，受抑制的半皮质中表达PV的神经元明显减少，但是与对照组相比，CTB标记的皮质脊髓神经元的数量也一样多，从对侧到植入物的皮质脊髓投射量也相同，明显大于对照组。出乎意料的是，单侧抑制运动皮质输入并没有导致肌肉传入输入的扩大。相反，随着双侧皮质脊髓神经支配的发展，这减少了。

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