...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Dynamic changes in glypican-1 expression in dorsal root ganglion neurons after peripheral and central axonal injury.
【24h】

Dynamic changes in glypican-1 expression in dorsal root ganglion neurons after peripheral and central axonal injury.

机译：周围和中央轴突损伤后背根神经节神经元中glypican-1表达的动态变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Glypican-1, a glycosyl phosphatidyl inositol (GPI)-anchored heparan sulphate proteoglycan expressed in the developing and mature cells of the central nervous system, acts as a coreceptor for diverse ligands, including slit axonal guidance proteins, fibroblast growth factors and laminin. We have examined its expression in primary sensory dorsal root ganglion (DRG) neurons and spinal cord after axonal injury. In noninjured rats, glypican-1 mRNA and protein are constitutively expressed at low levels in lumbar DRGs. Sciatic nerve transection results in a two-fold increase in mRNA and protein expression. High glypican-1 expression persists until the injured axons reinnervate their peripheral targets, as in the case of a crushed nerve. Injury to the central axons of DRG neurons by either a dorsal column injury or a dorsal root transection also up-regulates glypican-1, a feature that differs from most DRG axonal injury-induced genes, whose regulation changes only after peripheral and not central axonal injury. After axonal injury, the cellular localization of glypican-1 changes from a nuclear pattern restricted to neurons in noninjured DRGs, to the cytoplasm and membrane of injured neurons, as well as neighbouring non-neuronal cells. Sciatic nerve transection also leads to an accumulation of glypican-1 in the proximal nerve segment of injured axons. Glypican-1 is coexpressed with robo 2 and its up-regulation after axonal injury may contribute to an altered sensitivity to axonal growth or guidance cues.
机译：摘要Glypican-1是糖基磷脂酰肌醇（GPI）锚定的硫酸乙酰肝素蛋白聚糖，在中枢神经系统的发育和成熟细胞中表达，可作为多种配体的共受体，包括缝隙轴突引导蛋白，成纤维细胞生长因子和层粘连蛋白。我们已经检查了其在轴突损伤后在初级感觉背根神经节（DRG）神经元和脊髓中的表达。在未受伤的大鼠中，glypican-1 mRNA和蛋白质在腰部DRG中低水平组成性表达。坐骨神经横切导致mRNA和蛋白质表达增加两倍。高glypican-1的表达一直持续到受伤的轴突重新激活其周围的目标，例如神经受压的情况。背柱损伤或背根横断对DRG神经元中央轴突的伤害也上调了glypican-1，这一特性不同于大多数DRG轴突损伤诱导基因，后者的调节仅在周围轴突而不是中央轴突后才改变受伤。轴突损伤后，glypican-1的细胞定位从限于非受伤DRG中神经元的核模式改变为受损神经元的细胞质和膜，以及邻近的非神经元细胞。坐骨神经横切还导致受损轴突的近端神经节中glypican-1的积累。 Glypican-1与robo 2共表达，轴突损伤后其上调可能导致对轴突生长或指导信号的敏感性改变。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号