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首页> 外文期刊>The European Journal of Neuroscience >Temporal and spatial expression patterns of canonical clock genes and clock-controlled genes in the suprachiasmatic nucleus.
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Temporal and spatial expression patterns of canonical clock genes and clock-controlled genes in the suprachiasmatic nucleus.

机译：视交叉上核中规范时钟基因和时钟控制基因的时空表达模式。

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Abstract In mammals, the suprachiasmatic nuclei (SCN) of the hypothalamus control endogenous circadian rhythms and entrainment to the environment. A core SCN region of calbindin (CalB)-containing cells is retinorecipient and the cells therein lack rhythmic expression of clock genes and electrical activity. The core is surrounded by a 'shell' of rhythmic oscillator cells. In the present experiments, we studied the spatial arrangement of oscillator cells by examining the spatial and temporal patterns of expression of the canonical clock genes Per1, Per2 and vasopressin mRNA, a clock-controlled gene. Surprisingly, in the SCN shell, the dorsomedial cells were the first to rhythmically express both Per1 and VP mRNA, with gene expression then spreading very slowly through much of the nucleus for the next 12 h then receding to baseline levels. Following a light pulse, Per expression increased after 1 h in the core SCN and after 1.5 h in the shell. Although expression in the shell occurred earlier in light-pulsed animals than in those housed in constant darkness, it still followed the same spatial and temporal expression pattern as was observed in constant darkness. The results suggest that not only is the SCN organized into light-responsive and rhythmic regions but also that the rhythmic region of the SCN itself has an ordered arrangement of SCN oscillator cells.
机译：摘要在哺乳动物中，下丘脑上眼睑上核（SCN）控制着内源性昼夜节律并带入环境。含有钙结合蛋白（CalB）的细胞的核心SCN区域是视网膜受体，其中的细胞缺乏时钟基因的节律表达和电活性。核心被有节奏的振荡器单元的“外壳”包围。在本实验中，我们通过检查规范时钟基因Per1，Per2和加压素mRNA（一种时钟控制基因）的表达的时空模式，研究了振荡器细胞的空间排列。出乎意料的是，在SCN壳中，背层细胞率先有节奏地表达Per1和VP mRNA，然后在接下来的12小时内，基因表达非常缓慢地扩散到大部分核中，然后退回到基线水平。受到光脉冲后，核心SCN中1小时后和壳中1.5小时后，Per表达增加。尽管光脉冲动物的壳中表达比恒定黑暗中的动物更早发生，但它仍遵循与恒定黑暗中观察到的相同的时空表达模式。结果表明，不仅SCN被组织成光响应性和有节奏的区域，而且SCN本身的有节奏的区域具有SCN振荡器单元的有序排列。

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