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首页> 外文期刊>The European Journal of Neuroscience >Pro-VGF-derived peptides induce penile erection in male rats: possible involvement of oxytocin.
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Pro-VGF-derived peptides induce penile erection in male rats: possible involvement of oxytocin.

机译：VGF衍生肽在雄性大鼠中诱发阴茎勃起：催产素可能参与其中。

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摘要

The effect of five peptides derived from the C-terminal portion of rat pro-VGF (VGF(577-617), VGF(588-617), VGF(599-617), VGF(556-576) and VGF(588-597)) on penile erection was studied after injection into the hypothalamic paraventricular nucleus of male rats. VGF(577-617), VGF(588-617), VGF(599-617) and, to a lower extent, VGF(588-597) (0.1-2 microg) induced penile erection episodes in a dose-dependent manner when injected into the paraventricular nucleus, while VGF(556-576) was ineffective. VGF(588-617)-induced penile erection was reduced by nitro(omega)-L-arginine methylester (L-NAME; 20 microg), by morphine (5 microg) and by muscimol (1 microg), but not by dizocilpine [(+)MK-801; 1 microg], nor by cis-flupenthixol (10 microg) given into the paraventricular nucleus 10 min before the VGF peptide. d(CH2)5Tyr(Me)-Orn8-vasotocin (1 microg) effectively reduced VGF(588-617)-induced penile erection when given into the lateral ventricles but not when injected into the paraventricular nucleus. Immunocytochemistry with antibodies specific for the C-terminal nonapeptide sequence of pro-VGF (VGF(609-617)) revealed numerous neuronal fibres and terminals within the paraventricular nucleus, including its parvocellular components. Here, many immunostained neuronal terminals impinged on parvocellular oxytocinergic neurons. The present results show for the first time that certain pro-VGF C-terminus-derived peptides promote penile erection when injected into the paraventricular nucleus and suggest that, within this nucleus, these or closely related pro-VGF-derived peptides may be released to influence sexual function by activating paraventricular oxytocinergic neurons mediating penile erection.
机译：大鼠前VGF（VGF（577-617），VGF（588-617），VGF（599-617），VGF（556-576）和VGF（588- 597））对雄性大鼠的下丘脑室旁核进行注射后，对阴茎勃起进行了研究。 VGF（577-617），VGF（588-617），VGF（599-617）以及较低程度的VGF（588-597）（0.1-2微克）以剂量依赖性方式诱导阴茎勃起VGF（556-576）无效时，将其注入脑室旁核。 VGF（588-617）引起的阴茎勃起可通过硝基（ω-）-L-精氨酸甲酯（L-NAME; 20微克），吗啡（5微克）和麝香酚（1微克）减少，但不会被二唑西平[ （+）MK-801; 1微克]，也不要在VGF肽之前10分钟将顺式氟戊醇（10微克）注入脑室旁核。当将d（CH2）5Tyr（Me）-Orn8-血管毒素（1微克）给予侧脑室时有效减少了VGF（588-617）引起的阴茎勃起，但当注入心室旁核时则没有。用对前VGF的C端九肽序列有特异性的抗体（VGF（609-617））进行的免疫细胞化学分析显示，脑室旁核内有许多神经元纤维和末端，包括其小细胞成分。在这里，许多免疫染色的神经元末梢撞击在细小细胞催产素能神经元上。目前的结果首次表明，某些前VGF C端衍生的肽在注射入室旁核时可促进阴茎勃起，并暗示在这些核内，这些或紧密相关的前VGF衍生肽可释放为通过激活介导阴茎勃起的室旁催产神经元来影响性功能。

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