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首页> 外文期刊>The European Journal of Neuroscience >Sex differences in adult suprachiasmatic nucleus neurons emerging late prenatally in rats.
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Sex differences in adult suprachiasmatic nucleus neurons emerging late prenatally in rats.

机译：在大鼠产前晚期出现的成年大鼠视交叉上核神经元的性别差异。

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Abstract The suprachiasmatic nucleus (SCN) is implicated in the control of circadian rhythms of gonadal function. Although several structures surrounding the SCN are sensitive to the effects of gonadal steroids, similar effects in the SCN remain unclear. For example, there are conflicting data on whether the SCN is sexually differentiated. This study attempted to determine sex differences in the number of SCN cells generated during late gestation, and if testosterone mediates these differences. Pregnant female rats were treated with 5-bromo-2'-deoxyuridine (BrdU; 50 mg/kg) on gestational day 18 (E18), the day when aromatase activity peaks in the developing rat fetus. These animals were also given injections of oil or testosterone propionate (10 mg/0.1 mL peanut oil) from E15 until parturition. Litters were allowed to survive until adulthood and were killed on postnatal day 60 (PN60). Following fixation, brain sections containing the SCN from these rats were processed for BrdU immunocytochemistry. A second set of SCN sections was processed for immunocytochemistry detecting BrdU and some of the cell groups prevalent within the SCN. Data showed that female rats have a higher number of cells labeled with BrdU in the SCN, particularly in the medial and caudal SCN. This sex difference was abolished in animals treated with testosterone during late gestation. Double immunocytochemistry revealed that BrdU-labeled cells were neurons expressing calbindin-D28K, vasoactive intestinal peptide and, to a lesser degree, vasopressin. Our results unveiled a previously unknown effect of gonadal steroids on the developing SCN, which may contribute to the emergence of gender-specific circadian rhythms.
机译：摘要视交叉上核（SCN）参与性功能昼夜节律的控制。尽管围绕SCN的几个结构对性腺类固醇的作用敏感，但尚不清楚SCN中的类似作用。例如，关于SCN是否在性别上存在冲突的数据。这项研究试图确定妊娠后期产生的SCN细胞数量的性别差异，以及睾丸激素是否介导了这些差异。在妊娠第18天（E18），即在发育中的大鼠胎儿中芳香化酶活性达到峰值的那一天，用5-溴-2'-脱氧尿苷（BrdU; 50 mg / kg）对怀孕的雌性大鼠进行治疗。从分娩前直到分娩前，还给这些动物注射油或丙酸睾丸酮（10 mg / 0.1 mL花生油）。凋落物可以存活到成年，并在出生后第60天（PN60）被杀死。固定后，将来自这些大鼠的含有SCN的脑切片进行BrdU免疫细胞化学处理。处理第二组SCN切片进行免疫细胞化学检测，检测BrdU和SCN中普遍存在的一些细胞群。数据显示，雌性大鼠在SCN中，特别是在内侧和尾部SCN中，具有被BrdU标记的细胞数量更高。在妊娠后期用睾丸激素治疗的动物中这种性别差异被消除。双重免疫细胞化学显示，BrdU标记的细胞是表达calbindin-D28K，血管活性肠肽和程度较低的血管加压素的神经元。我们的研究结果揭示了性腺类固醇对发育中的SCN的未知作用，这可能有助于出现性别特定的昼夜节律。

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